caa a22 4500 445803762 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10337-011-2054-y doi (NATIONALLICENCE)springer-10.1007/s10337-011-2054-y LC-MS-MS Determination of Gizzerosine in Poultry Feed [Elektronische Daten] [Silvio Uhlig, Magne Kaldhusdal, Anne-Gerd Gjevre] A new selective method for the determination of the bioactive amine gizzerosine in poultry feed is presented based on hydrophilic interaction chromatography coupled to electrospray ionisation and tandem quadrupole mass spectrometry. The protonated molecular ions were selected for fragmentation and scanning of the two major product ions using the mass analyser in the multiple reaction monitoring mode. The analytical methodology included acid hydrolysis of feed sample aliquots and desalting and crude clean-up of hydrolysates using cation-exchange chromatography on a polymeric solid-phase extraction sorbent. Mean overall recoveries (n=9) were 68-82% in spike-recovery experiments. The limit of detection was 0.25mgkg−1. The method was applied to the analysis of fish meal and fish meal-containing poultry feed samples. Although gizzerosine could not be detected in any of the samples, it was found in fish meal made from sand eel after heating to 130°C for 24h. Springer-Verlag, 2011 HILIC nationallicence Mass spectrometry nationallicence Amine nationallicence Feed nationallicence Gizzard erosion nationallicence Uhlig Silvio Section for Chemistry and Toxicology, Norwegian Veterinary Institute, Sentrum, P.O. Box 750, 0106, Oslo, Norway aut Kaldhusdal Magne Section for Pathology, Norwegian Veterinary Institute, Sentrum, P.O. Box 750, 0106, Oslo, Norway aut Gjevre Anne-Gerd Section for Pathology, Norwegian Veterinary Institute, Sentrum, P.O. Box 750, 0106, Oslo, Norway aut Chromatographia Springer-Verlag 74/1-2(2011-07-01), 133-138 0009-5893 74:1-2<133 2011 74 10337 https://doi.org/10.1007/s10337-011-2054-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2054-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uhlig Silvio Section for Chemistry and Toxicology, Norwegian Veterinary Institute, Sentrum, P.O. Box 750, 0106, Oslo, Norway aut NATIONALLICENCE 700 1- Kaldhusdal Magne Section for Pathology, Norwegian Veterinary Institute, Sentrum, P.O. Box 750, 0106, Oslo, Norway aut NATIONALLICENCE 700 1- Gjevre Anne-Gerd Section for Pathology, Norwegian Veterinary Institute, Sentrum, P.O. Box 750, 0106, Oslo, Norway aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/1-2(2011-07-01), 133-138 0009-5893 74:1-2<133 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer