caa a22 4500 445803770 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10337-011-2020-8 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2020-8 Simultaneous Quantification of Oroxylin A and Its Metabolite Oroxylin A-7- O -Glucuronide: Application to a Pharmacokinetic Study in Rat [Elektronische Daten] [Wenyuan Liu, Xiaozhen Xu, Feng Feng, Chunyong Wu] Oroxylin A shows reversal activity on multidrug resistance and has an attractive safety profile. A highly sensitive and specific method based on liquid chromatography coupled with tandem mass spectroscopy was developed and validated for simultaneous determination of oroxylin A and its major metabolite, oroxylin A-7-O-glucuronide in rat plasma. The assay procedure involved precipitation of plasma samples with acetonitrile after adding luteolin as an internal standard. Chromatographic separation was achieved on a C18 column using methanol and 0.5% formic acid as the mobile phase, eluting in a gradient system. Detection was achieved with selected reaction monitoring using the electrospray ionization technique. The method was validated by evaluation of selectivity, linearity, precision, accuracy and limit of quantification. Its applicability was demonstrated through the quantification of oroxylin A and oroxylin A-7-O-glucuronide in plasma after intravenous administration of oroxylin A at a dose of 20mgkg−1 to rats. The developed quantification method can now be used for pharmacokinetic studies after intravenous infusion of oroxylin A injection in rats. Springer-Verlag, 2011 Liquid chromatography nationallicence Tandem mass spectrometry nationallicence Pharmacokinetic study nationallicence Oroxylin A nationallicence Oroxylin A-7- O -glucuronide nationallicence Liu Wenyuan Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut Xu Xiaozhen Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut Feng Feng Department of Phytochemistry, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut Wu Chunyong Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut Chromatographia Springer-Verlag 74/1-2(2011-07-01), 75-81 0009-5893 74:1-2<75 2011 74 10337 https://doi.org/10.1007/s10337-011-2020-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2020-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wenyuan Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Xu Xiaozhen Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Feng Feng Department of Phytochemistry, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Wu Chunyong Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/1-2(2011-07-01), 75-81 0009-5893 74:1-2<75 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer