caa a22 4500 445803789 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10337-011-2037-z doi (NATIONALLICENCE)springer-10.1007/s10337-011-2037-z Clinic Pharmacokinetic Profiles of Huperzine A Following Transdermal Administration to Healthy Human Volunteers [Elektronische Daten] [Ting Wu, Chang-Yin Li, Min Chen, Jun Zhang, Heng-Shan Tan, Chen Yang, Wen-Zheng Ju] To support the rationale behind the development of the huperzine A patches and its pharmacological effects in clinical therapy, an LC-MS-MS method was developed and validated for determination of huperzine A in human plasma and then applied to the clinic pharmacokinetics study on transdermal patches of huperzine A. After a simple liquid-liquid extraction with ethyl acetate, analytes were separated on a C18 column with isocratic elution. The detection of analytes was performed on a tandem mass system equipped with an electrospray ionization source in positive mode using multiple-reaction monitoring. The MS-MS ion transitions monitored were m/z 243.2→210.1 for huperzine A and m/z 248.1→128.0 for tinidazole (internal standard). Method validation and sample analysis were performed according to FDA guidelines and the results met the acceptance criteria. The pharmacokinetic profiles of huperzine A following single transdermal administration of 8, 10 and 12mg huperzine A to healthy human volunteers were depicted using the established method. The results showed that the pharmacokinetic behavior of huperzine A in vivo was present as linear dynamic characteristics with much longer T max, lower C max, and relatively constant plasma concentration. No gender difference and obvious adverse effects were observed from all three groups in our study. Our study indicated that the huperzine A transdermal patches could provide continuous drug delivery over 120h with favorable tolerability. This may allow patients to obtain optimal plasma concentrations of drugs and to benefit from a longer duration of treatment. Springer-Verlag, 2011 Column-liquid chromatography-mass spectrometry nationallicence Huperzine A nationallicence Transdermal patch nationallicence Pharmacokinetic nationallicence Human plasma nationallicence Wu Ting Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut Li Chang-Yin Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut Chen Min Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut Zhang Jun Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut Tan Heng-Shan Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut Yang Chen Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut Ju Wen-Zheng Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut Chromatographia Springer-Verlag 74/1-2(2011-07-01), 67-73 0009-5893 74:1-2<67 2011 74 10337 https://doi.org/10.1007/s10337-011-2037-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2037-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Ting Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Li Chang-Yin Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Chen Min Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Zhang Jun Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Tan Heng-Shan Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Yang Chen Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut NATIONALLICENCE 700 1- Ju Wen-Zheng Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, 210029, Nanjing, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/1-2(2011-07-01), 67-73 0009-5893 74:1-2<67 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer