caa a22 4500 445803835 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10337-011-2042-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2042-2 LC-MS-MS Quantitative Determination of Gefitinib in Human Serum and Cerebrospinal Fluid [Elektronische Daten] [Xin Zheng, Pei Hu, Jian Liu, Qian Zhao, Mengzhao Wang, Ji Jiang, Wei Zhong] A specific, sensitive, and rapid method based on high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS) was developed for determination of gefitinib in human serum and cerebrospinal fluid (CSF). The analyte was detected by tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring (MRM). Gefitinib was extracted from serum or CSF samples with ethyl acetate using icotinib as internal standard. The method was validated over the concentration range of 1.00-1,000ngmL−1 in human serum and 0.05-50.0ngmL−1 in CSF. For both matrices, inter- and intraday precision (CV%) were less than 15% and accuracy was within 85-115%. Average extraction recoveries were 78.9 and 61.8% in human serum and CSF, respectively. Linearity, recovery, matrix effects, and stability were validated in the two matrices. The method was successfully used for analysis of clinical samples from lung cancer patients with brain metastases treated with gefitinib in the dosage range of 250-500mgday−1. Springer-Verlag, 2011 LC-MS-MS nationallicence Gefitinib nationallicence Human serum nationallicence CSF nationallicence Brain metastases nationallicence Zheng Xin Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut Hu Pei Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut Liu Jian Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut Zhao Qian Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut Wang Mengzhao Department of Respiratory Medicine, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut Jiang Ji Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut Zhong Wei Department of Respiratory Medicine, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut Chromatographia Springer-Verlag 74/1-2(2011-07-01), 41-49 0009-5893 74:1-2<41 2011 74 10337 https://doi.org/10.1007/s10337-011-2042-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2042-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zheng Xin Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut NATIONALLICENCE 700 1- Hu Pei Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut NATIONALLICENCE 700 1- Liu Jian Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut NATIONALLICENCE 700 1- Zhao Qian Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut NATIONALLICENCE 700 1- Wang Mengzhao Department of Respiratory Medicine, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut NATIONALLICENCE 700 1- Jiang Ji Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut NATIONALLICENCE 700 1- Zhong Wei Department of Respiratory Medicine, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, 100730, Beijing, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/1-2(2011-07-01), 41-49 0009-5893 74:1-2<41 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer