caa a22 4500 445803851 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10337-011-2021-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2021-7 Comparison of LC Columns Packed with 2.6μm Core-Shell and Sub-2μm Porous Particles for Gradient Separation of Antibiotics [Elektronische Daten] [Tereza Tylová, Zdeněk Kameník, Miroslav Flieger, Jana Olšovská] The recently introduced Kinetex C18 column packed with core-shell 2.6μm particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7μm porous particles. Unlike Acquity BEH C18 column, Kinetex C18 column exhibited lower column backpressure making this column compatible to conventional LC systems. The performance of Kinetex C18 column (2.1×50mm) and Acquity BEH C18 column (2.1×50mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope. The maximal experimental peak capacities for analysis of tetracyclines were 51.8 (Acquity BEH C18 column) and 48.4 (Kinetex C18 column). This indicated that Kinetex C18 was a suitable alternative to Acquity BEH C18 column for the analysis of tetracyclines under acidic conditions. On the contrary, the maximal experimental peak capacities for analysis of macrolides on Acquity BEH C18 column was higher (46.7) than that on Kinetex C18 column (36.9). Moreover, application of Kinetex C18 column for the analysis of macrolides under alkaline conditions was limited with respect to its decreasing performance with growing number of injections on the column. Springer-Verlag, 2011 Column liquid chromatography nationallicence Sub-2μm particles nationallicence Acquity BEH C18 column nationallicence Core-shell particles nationallicence Kinetex C18 column nationallicence Macrolides nationallicence Tetracyclines nationallicence Tylová Tereza Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut Kameník Zdeněk Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut Flieger Miroslav Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut Olšovská Jana Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut Chromatographia Springer-Verlag 74/1-2(2011-07-01), 19-27 0009-5893 74:1-2<19 2011 74 10337 https://doi.org/10.1007/s10337-011-2021-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2021-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tylová Tereza Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Kameník Zdeněk Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Flieger Miroslav Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Olšovská Jana Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/1-2(2011-07-01), 19-27 0009-5893 74:1-2<19 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer