caa a22 4500 445803924 CHVBK 20180317145151.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10337-011-2035-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2035-1 Determination of Imidol in Rat Plasma by UPLC-MS-MS and Its Application in a Pharmacokinetic Study [Elektronische Daten] [Zhong-Bo Liu, Qing Li, Wei Li, Xiao-Wei Song, Guang-Ping Rong, Yan-Fang Zhao, Ping Gong, Kai-Shun Bi, Xiao-Hui Chen] A rapid, sensitive and accurate ultra-performance liquid chromatography/tandem mass spectrometry method was developed and validated for the quantitative determination of imidol in rat plasma for the first time. The analyte and internal standard were extracted from plasma by liquid-liquid extraction with diethyl ether. The separation was performed on a BEH C18 column (50mm×2.1mm, 1.7μm). The detection was carried out by electrospray ionization mass spectrometry in positive ion mode with multiple reaction monitoring. Linear calibration curves were obtained in the concentration range of 2.5-2,500ngmL−1, with the lower limit of quantification of 2.5ngmL−1. The intra- and inter-day precision (RSD) values were below 8% and accuracy (RE) was from −7.9 to 6.3%. After strict validation, the method was applied successfully to the pharmacokinetic study of imidol in rats after oral and intravenous administration, respectively. Springer-Verlag, 2011 UPLC-MS-MS nationallicence Imidol nationallicence HBV nationallicence Rat plasma nationallicence Pharmacokinetics nationallicence Liu Zhong-Bo School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Li Qing School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Li Wei School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Song Xiao-Wei School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Rong Guang-Ping School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Zhao Yan-Fang School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Gong Ping School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Bi Kai-Shun School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Chen Xiao-Hui School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Chromatographia Springer-Verlag 74/1-2(2011-07-01), 59-66 0009-5893 74:1-2<59 2011 74 10337 https://doi.org/10.1007/s10337-011-2035-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2035-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Zhong-Bo School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Li Qing School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Li Wei School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Song Xiao-Wei School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Rong Guang-Ping School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Zhao Yan-Fang School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Gong Ping School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Bi Kai-Shun School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Chen Xiao-Hui School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/1-2(2011-07-01), 59-66 0009-5893 74:1-2<59 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer