caa a22 4500 445803983 CHVBK 20180317145151.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10337-011-2145-9 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2145-9 UPLC-TOF MS-Based Metabonomic Study on Coadministration of Huperzine A and Ligustrazine Phosphate for Treatment of Alzheimer's Disease [Elektronische Daten] [Jun Shi, Yiming Wang, Guoan Luo] This work was designed to assess the holistic efficacy of concurrent treatment with huperzine A (HA) and ligustrazine phosphate (LP) in the animal model, and to interpret the metabonomics characteristics of the poly-therapy in treating Alzheimer's disease (AD). Metabolic profiling of the brain was performed using ultra performance liquid chromatography coupled to time-of-flight mass spectroscopy (UPLC-TOF MS). Principal component analysis and partial least squares discriminant analysis were utilized to classify and reveal the differences among the control, model and treatment groups. Ten potential biomarkers have been finally gained. The metabonomic results revealed that the coadministration of HA and LP might slow down the progression of AD through synergistic therapeutic efficacies, exhibiting better effect on amnesia compared with the mono-drug therapy. Importantly, the UPLC-TOF MS-based metabonomic approach would be a useful technique for the diagnosis of AD, the discovery of metabolic biomarkers and the evaluation of the treatment effects. Springer-Verlag, 2011 UPLC-TOF MS nationallicence Huperzine A nationallicence Ligustrazine phosphate nationallicence Alzheimer's disease nationallicence Synergistic effect nationallicence Shi Jun School of Pharmacy, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Wang Yiming Department of Chemistry, Tsinghua University, 100084, Beijing, People's Republic of China aut Luo Guoan School of Pharmacy, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Chromatographia Springer-Verlag 74/11-12(2011-12-01), 827-832 0009-5893 74:11-12<827 2011 74 10337 https://doi.org/10.1007/s10337-011-2145-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2145-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shi Jun School of Pharmacy, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yiming Department of Chemistry, Tsinghua University, 100084, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Luo Guoan School of Pharmacy, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/11-12(2011-12-01), 827-832 0009-5893 74:11-12<827 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer