caa a22 4500 445804041 CHVBK 20180317145151.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10337-011-2147-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2147-7 Simultaneous Determination of High-Density Lipoprotein, Very Low-Density Lipoprotein and Low-Density Lipoprotein Subclass in Human Serum by Microchip CE [Elektronische Daten] [Hua Wang, Chongxu Han, Huimin Wang, Qinghui Jin, Daxin Wang, Li Cao, Guangzhou Wang] Lipoproteins, especially high-density lipoproteins (HDL), very low-density lipoproteins (VLDL) and small, dense low-density lipoprotein (sdLDL), are believed to play an important role in the development of atherosclerosis. In this work, a simple, selective and sensitive method for the simultaneous monitoring of these lipoproteins in human serum using microchip capillary electrophoresis was developed. Gold nanoparticles were used as an additive to the running buffer to obtain the absolute separation of the lipoproteins. Under optimised conditions, the linear ranges of large buoyant low-density lipoproteins, sdLDL, VLDL and HDL were 10-800, 10-800, 40-1,000 and 20-800μgL−1, and their limits of detection were 5, 5, 15 and 8μgL−1, respectively. The intraassay and interassay relative standard deviation of lipoprotein peak areas were in the range of 3.8-7.4%. For practical application, variations in the serum lipoprotein of coronary heart disease patients were monitored by microchip-based CE. The results showed that the method was applicable for routine clinical use and allowed the rapid detection of different lipoprotein classes as well as their subclasses, thus greatly improving the analysis of atherosclerotic risk factors. Springer-Verlag, 2011 Microchip capillary electrophoresis nationallicence Gold nanoparticles nationallicence High-density lipoprotein nationallicence Very low-density lipoprotein nationallicence Small, dense low-density lipoprotein nationallicence Wang Hua Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut Han Chongxu Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut Wang Huimin Affiliated Hospital of Nantong University, 20 Xi Si Road, 226001, Nantong, Jiangsu, China aut Jin Qinghui Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, 865 Chang Ning Road, 200050, Shanghai, China aut Wang Daxin Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut Cao Li Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut Wang Guangzhou Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut Chromatographia Springer-Verlag 74/11-12(2011-12-01), 799-805 0009-5893 74:11-12<799 2011 74 10337 https://doi.org/10.1007/s10337-011-2147-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2147-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Hua Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Han Chongxu Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Wang Huimin Affiliated Hospital of Nantong University, 20 Xi Si Road, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Jin Qinghui Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, 865 Chang Ning Road, 200050, Shanghai, China aut NATIONALLICENCE 700 1- Wang Daxin Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Cao Li Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Wang Guangzhou Clinical Medical College of Yangzhou University, 98 Nangtong West Road, 200050, Yangzhou, Jiangsu, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/11-12(2011-12-01), 799-805 0009-5893 74:11-12<799 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer