caa a22 4500 44580405X CHVBK 20180317145151.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10337-011-2142-z doi (NATIONALLICENCE)springer-10.1007/s10337-011-2142-z Simultaneous Determination of Zoalene and Its Metabolite in Chicken Muscle and Liver by SPE and UPLC-MS-MS [Elektronische Daten] [Yin-Liang Wu, Yong Xu, Ting Yang, Wei-Guo Huang-Fu] This paper presents an analytical method for the simultaneous determination of zoalene and its metabolite 3-amino-5-nitro-o-toluamide (3-ANOT) in chicken muscle and liver by solid phase extraction and UPLC-MS-MS operated in the positive and negative ionization switching mode. Samples were extracted with phosphate buffer solution and purified with OASIS™ HLB cartridge after pH adjustment. The determination was carried out using UPLC-MS-MS on a Waters Acquity BEH C18 column with 0.1% formic acid in water/acetonitrile as mobile phase with gradient elution. The linearity of the analytical response across the studied range of concentrations (2.0-1,000μgL−1) was excellent, obtaining correlation coefficients higher than 0.999. Matrix effects had been investigated for zoalene and 3-ANOT. Recovery studies were carried out on spiked chicken muscle and liver blank samples, at four concentration levels (50, 1,500, 3,000, and 4,500μgkg−1 for chicken muscle and 50, 3,000, 6,000, and 9,000μgkg−1 for chicken liver) performing six replicates at each level. Mean recoveries of 77.9-94.2% with CVs of 3.2-8.7% were obtained. The method demonstrated to be suitable for the simultaneous determination of zoalene and 3-ANOT in chicken tissues. Springer-Verlag, 2011 Zoalene nationallicence 3-ANOT nationallicence Chicken tissues nationallicence Column liquid chromatography-electrospray tandem mass spectrometry nationallicence Solid phase extraction nationallicence Wu Yin-Liang The Ningbo Academy of Agricultural Sciences, 315040, Ningbo, People's Republic of China aut Xu Yong Institute for the Control of Agro-chemicals of Zhejiang Province, 310020, Hangzhou, People's Republic of China aut Yang Ting The Ningbo Academy of Agricultural Sciences, 315040, Ningbo, People's Republic of China aut Huang-Fu Wei-Guo The Ningbo Academy of Agricultural Sciences, 315040, Ningbo, People's Republic of China aut Chromatographia Springer-Verlag 74/11-12(2011-12-01), 833-838 0009-5893 74:11-12<833 2011 74 10337 https://doi.org/10.1007/s10337-011-2142-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2142-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Yin-Liang The Ningbo Academy of Agricultural Sciences, 315040, Ningbo, People's Republic of China aut NATIONALLICENCE 700 1- Xu Yong Institute for the Control of Agro-chemicals of Zhejiang Province, 310020, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Ting The Ningbo Academy of Agricultural Sciences, 315040, Ningbo, People's Republic of China aut NATIONALLICENCE 700 1- Huang-Fu Wei-Guo The Ningbo Academy of Agricultural Sciences, 315040, Ningbo, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/11-12(2011-12-01), 833-838 0009-5893 74:11-12<833 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer