caa a22 4500 445804076 CHVBK 20180317145151.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10337-011-2140-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2140-1 Simultaneous RP-HPLC Determination of Six Platycosides: Application to an Enzymatic Preparation Study [Elektronische Daten] [Wei Li, Ming Zhang, Zi Wang, Ying Wang, Yi-nan Zheng, Jing Zhang] Platycosides, main pharmacological effective compounds, are known to have several biological activities, including anti-obesity, anti-cancer and anti-diabetes. Although enzymatic preparation of platycosides was considered as effective method to obtain them, few analytical methods have been reported on process control. In the present study, we developed an application of reversed-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of six platycosides during the process of enzymatic preparation of deapio-platycodin D (dPD) and platycodin D (PD). The method employed a Hypersil ODS2 analytical column (250×4.6mm I.D., 5μm) coupled with UV detector at 210nm with flow rate of 1.0mgmL−1. A step gradient of acetonitrile-water (v/v) was applied, leading to a sample analysis of 60min. The method was validated in terms of linearity, sensitivity, precision and accuracy. The correlation coefficients (R 2) for calibration curves of platycosides were in the range of 0.9995-1.0 when the linearity range was from 0.85 to 10.2mgmL−1. The proposed RP-HPLC method was successfully applied to the analysis of enzymatic preparation study and the recoveries of platycosides were in the range of 96.22-102.56% with RSD <3.3%. The method could be of use for rapid and routine evaluation of the quantity of platycosides during the enzymatic preparation process. Springer-Verlag, 2011 RP-HPLC nationallicence Platycosides nationallicence Enzymatic preparation nationallicence Radix platycodi nationallicence Li Wei College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut Zhang Ming Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut Wang Zi College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut Wang Ying Institute of Special Wild Economic Animals and Plant, CAAS, 132109, Jilin, People's Republic of China aut Zheng Yi-nan College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut Zhang Jing College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut Chromatographia Springer-Verlag 74/11-12(2011-12-01), 777-782 0009-5893 74:11-12<777 2011 74 10337 https://doi.org/10.1007/s10337-011-2140-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2140-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Wei College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ming Norman Bethune College of Medicine, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zi College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wang Ying Institute of Special Wild Economic Animals and Plant, CAAS, 132109, Jilin, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Yi-nan College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jing College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street, No. 2888, 1300118, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/11-12(2011-12-01), 777-782 0009-5893 74:11-12<777 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer