caa a22 4500 445804106 CHVBK 20180317145151.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10337-011-2132-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2132-1 OH· Radical Production Induces Direct Enhancement of the Amyloïd β Protein/Chondroitin Sulfate Binding: Inhibition by Potentially Radical Scavengers, a Biochromatographic and Thermodynamic Model [Elektronische Daten] [Claire André, Nadine Magy-Bertrand, Yves Guillaume] β-Amyloid (Aβ) is a major component of the senile plaques characteristic of Alzheimer disease (AD). Chondroitin sulfate (CS) and glycoaminoglycan (GAG) are also localized throughout the senile plaques in AD. In previous studies, the interaction of the Aβ protein with CS immobilized on a chromatographic support and the role of aluminum and copper cations was studied using a molecular chromatographic approach [1, 2]. Here, we demonstrated the direct implication of OH· radical formation on this binding via a novel analytical procedure. The binding of Aβ amyloid on CS was accompanied by an OH· radical uptake. The Aβ-CS complex was stabilized by the OH· radical via the creation of about one to two hydrogen bonds. The addition in the medium of a radical scavenger allowed decreasing the Aβ/CS association and thus confirmed the positive role of these compounds in amyloidosis. Springer-Verlag, 2011 Liquid chromatography nationallicence OH· radical nationallicence Chondroitin sulfate nationallicence Fenton reagent nationallicence Thermodynamic nationallicence André Claire EA4267 Equipe Bio Analytique Synthèse, 25000, Besançon, France aut Magy-Bertrand Nadine EA4267 Equipe Bio Analytique Synthèse, 25000, Besançon, France aut Guillaume Yves EA4267 Equipe Bio Analytique Synthèse, 25000, Besançon, France aut Chromatographia Springer-Verlag 74/9-10(2011-11-01), 711-715 0009-5893 74:9-10<711 2011 74 10337 https://doi.org/10.1007/s10337-011-2132-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2132-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- André Claire EA4267 Equipe Bio Analytique Synthèse, 25000, Besançon, France aut NATIONALLICENCE 700 1- Magy-Bertrand Nadine EA4267 Equipe Bio Analytique Synthèse, 25000, Besançon, France aut NATIONALLICENCE 700 1- Guillaume Yves EA4267 Equipe Bio Analytique Synthèse, 25000, Besançon, France aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/9-10(2011-11-01), 711-715 0009-5893 74:9-10<711 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer