caa a22 4500 445804203 CHVBK 20180317145152.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10337-011-2133-0 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2133-0 El Deeb Sami Institute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany aut Monolithic Silica for Fast HPLC: Current Success and Promising Future [Elektronische Daten] [Sami El Deeb] As an approach for fast HPLC, monolithic silica has proven to be highly effective. It is especially successful for routinely obtaining fast isocratic HPLC analyses of small drug molecules. The low cost of monolithic compared with other approaches, such as UPLC, makes it more convenient for everyday application. It is also the more developed and widely applied technique compared with superficially porous particles. It offers the possibility for gaining high plate numbers through column coupling, but not at the expense of run time if a proper flow program is subsequently applied. Good precision and batch reproducibility are now achieved with commercially available monolithic silica columns. The application of monolithic silica columns is already well developed in various fields. It invades the field of bio-analysis and proteomics. Hundreds of analytical methods have already been successfully transferred to or developed on monolithic silica columns. An updated strategy based on Snyder's method for rapid method development using monolithic column has been provided. However, more is still to be expected from monolithic silica in term of chemistry variation, application, and instrument compatibility. The future of monolithic silica is promising when considering the high demand for fast chromatographic analysis. Springer-Verlag, 2011 Fast HPLC nationallicence Monolithic silica nationallicence Fast analysis nationallicence Flow programming nationallicence Chromatographia Springer-Verlag 74/9-10(2011-11-01), 681-691 0009-5893 74:9-10<681 2011 74 10337 https://doi.org/10.1007/s10337-011-2133-0 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2133-0 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- El Deeb Sami Institute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/9-10(2011-11-01), 681-691 0009-5893 74:9-10<681 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer