caa a22 4500 445804211 CHVBK 20180317145152.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10337-011-2129-9 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2129-9 Preparation of Monolithic Imprinted Stationary Phase for Clenbuterol by In Situ Polymerization and Application in Biological Samples Pretreatment [Elektronische Daten] [Yifen Luo, Ping Huang, Qiang Fu, Wei Du, Sijuan Sun, Ya Li, Meng Liu, Chun Chang] A clenbuterol (CLB) molecule-imprinted monolithic stationary phase (MIMSP) with specific recognition for CLB and some other β2-adrenergic receptor agonists was prepared by in situ polymerization technique utilizing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linking reagent, and low polar solvents (toluene and dodecanol) as porogenic solvents. The optimal polymerization conditions were as follows: the molar ratio of template:monomer:initiator was 5:20:1, EDMA was 85% (v/v) in the total volume of monomer and EDMA, and toluene was 18% (v/v) in the total mixed porogen. The selectivity of the stationary phase for CLB and other β2-adrenergic receptor agonists was evaluated by high performance liquid chromatography. Scatchard analysis was employed to explore the recognition mechanism. Then the CLB-MIMSP was used as a solid phase extraction (SPE) stationary phase for concentration and purification of CLB from pig liver samples. The results showed that the obtained CLB-MIMSP possessed high selectivity towards CLB and moderate selectivity towards some other β2-adrenergic receptor agonists with characteristics of easy-made. The limit of detection was 10ngg−1, and recoveries of CLB were 99.16-113.06% with RSD 4.55-11.81% for the spiked pig liver samples. The CLB-MIMSP could be a promising SPE absorbent in CLB biological sample pretreatment. Springer-Verlag, 2011 Column liquid chromatography nationallicence Clenbuterol nationallicence Molecule-imprinted monolithic stationary phase nationallicence In situ polymerization nationallicence Solid phase extraction nationallicence Biological samples pretreatment nationallicence Luo Yifen Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Huang Ping Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Fu Qiang Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Du Wei Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Sun Sijuan Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Li Ya Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Liu Meng Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Chang Chun Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut Chromatographia Springer-Verlag 74/9-10(2011-11-01), 693-701 0009-5893 74:9-10<693 2011 74 10337 https://doi.org/10.1007/s10337-011-2129-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2129-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Yifen Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Huang Ping Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Fu Qiang Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Du Wei Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Sun Sijuan Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Li Ya Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Liu Meng Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Chang Chun Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/9-10(2011-11-01), 693-701 0009-5893 74:9-10<693 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer