caa a22 4500 44580422X CHVBK 20180317145152.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10337-011-2128-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-2128-x Simple, Sensitive, High-Throughput Method for the Quantification of Mitragynine in Rat Plasma Using UPLC-MS and Its Application to an Intravenous Pharmacokinetic Study [Elektronische Daten] [Pradeep Vuppala, Sai Boddu, Edward Furr, Christopher McCurdy, Bonnie Avery] A simple, sensitive and rapid ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method was developed and validated for the quantification of mitragynine in rat plasma using amitriptyline hydrochloride as an internal standard. Sample preparation involved a one-step liquid-liquid extraction using methyl t-butyl ether. Mitragynine was separated on an Acquity UPLC™ BEH HILIC column using isocratic elution with a mobile phase of 10mM ammonium formate buffer containing 0.1% formic acid:acetonitrile (15:85, v/v). At a flow rate of 0.2mLmin−1, the retention time of mitragynine was found to be 1.3min. Ionization was performed in the positive ion electrospray mode. The selected mass-to-charge (m/z) ratio transition of mitragynine ion [M+H]+ used in the selected ion recording (SIR) was 399.1. The calibration curve was found to be linear over a concentration range of 1-5,000ngmL−1 (r=0.999) with a lower limit of quantification (LLOQ) of 1ngmL−1. Intra- and inter-day assay variations were found to be less than 15%. The extraction recoveries ranged from 85-93% at the three concentrations (2, 400 and 4,000ngmL−1) in rat plasma. This method was successfully used to quantify mitragynine in rat plasma following intravenous administration of the compound. Springer-Verlag, 2011 UPLC-MS nationallicence High-throughput method nationallicence Mitragynine nationallicence Kratom nationallicence Rat plasma nationallicence Pharmacokinetics nationallicence Vuppala Pradeep Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut Boddu Sai Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut Furr Edward Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut McCurdy Christopher Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut Avery Bonnie Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut Chromatographia Springer-Verlag 74/9-10(2011-11-01), 703-710 0009-5893 74:9-10<703 2011 74 10337 https://doi.org/10.1007/s10337-011-2128-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2128-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vuppala Pradeep Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut NATIONALLICENCE 700 1- Boddu Sai Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut NATIONALLICENCE 700 1- Furr Edward Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut NATIONALLICENCE 700 1- McCurdy Christopher Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut NATIONALLICENCE 700 1- Avery Bonnie Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/9-10(2011-11-01), 703-710 0009-5893 74:9-10<703 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer