caa a22 4500 445810246 CHVBK 20180317145209.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00415-010-5825-8 doi (NATIONALLICENCE)springer-10.1007/s00415-010-5825-8 Monitoring systemic oxidative stress in an animal model of amyotrophic lateral sclerosis [Elektronische Daten] [Francisco Miana-Mena, Cristina González-Mingot, Pilar Larrodé, María Muñoz, Sara Oliván, Lorena Fuentes-Broto, Enrique Martínez-Ballarín, Russel Reiter, Rosario Osta, Joaquín García] A mutant form of the ubiquitous copper/zinc superoxide dismutase (SOD1) protein has been found in some patients with amyotrophic lateral sclerosis (ALS). We monitored oxidative stress in an animal model of ALS, the SODG93A mouse, which develops a disease similar to ALS with an accelerated course. The aim of this work was to show that ALS damages several organs and tissues, from an oxidative stress point of view. We measured lipid and protein oxidative damage in different tissue homogenates of SODG93A mice. The biomarkers that we analyzed were malondialdehyde+4-hydroxyalkenal (MDA+4-HDA) and carbonyls, respectively. The spinal cord and brain of SODG93A mice showed increased lipid peroxidation after 100 or 130days compared to age-matched littermate controls. The CNS was most affected, but lipid peroxidation was also detected in the skeletal muscle and liver on day 130. No changes were observed in protein carbonylation in the homogenates. Our results are consistent with a multisystem etiology of ALS and suggest that oxidative stress may play a primary role in ALS pathogenesis. Thus, oxidative stress represents a potential biomarker that might be useful in developing new therapeutic strategies for ALS. Springer-Verlag, 2010 Amyotrophic lateral sclerosis nationallicence Oxidative stress nationallicence G93A nationallicence Lipid peroxidation nationallicence Protein oxidation nationallicence Miana-Mena Francisco Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut González-Mingot Cristina Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut Larrodé Pilar Neurology Service, Universitary Hospital Lozano Blesa of Zaragoza, Zaragoza, Spain aut Muñoz María Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut Oliván Sara LAGENBIO, Universidad de Zaragoza, Zaragoza, Spain aut Fuentes-Broto Lorena Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut Martínez-Ballarín Enrique Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut Reiter Russel Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, USA aut Osta Rosario LAGENBIO, Universidad de Zaragoza, Zaragoza, Spain aut García Joaquín Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut Journal of Neurology Springer-Verlag 258/5(2011-05-01), 762-769 0340-5354 258:5<762 2011 258 415 https://doi.org/10.1007/s00415-010-5825-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00415-010-5825-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miana-Mena Francisco Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut NATIONALLICENCE 700 1- González-Mingot Cristina Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut NATIONALLICENCE 700 1- Larrodé Pilar Neurology Service, Universitary Hospital Lozano Blesa of Zaragoza, Zaragoza, Spain aut NATIONALLICENCE 700 1- Muñoz María Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut NATIONALLICENCE 700 1- Oliván Sara LAGENBIO, Universidad de Zaragoza, Zaragoza, Spain aut NATIONALLICENCE 700 1- Fuentes-Broto Lorena Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut NATIONALLICENCE 700 1- Martínez-Ballarín Enrique Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut NATIONALLICENCE 700 1- Reiter Russel Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, USA aut NATIONALLICENCE 700 1- Osta Rosario LAGENBIO, Universidad de Zaragoza, Zaragoza, Spain aut NATIONALLICENCE 700 1- García Joaquín Departamento Farmacología y Fisiología, Universidad de Zaragoza, c) Domingo Miral s/n, 50009, Zaragoza, Spain aut NATIONALLICENCE 773 0- Journal of Neurology Springer-Verlag 258/5(2011-05-01), 762-769 0340-5354 258:5<762 2011 258 415 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer