caa a22 4500 445810815 CHVBK 20180317145211.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00415-010-5738-6 doi (NATIONALLICENCE)springer-10.1007/s00415-010-5738-6 Tau and 14-3-3 of genetic and sporadic Creutzfeldt-Jakob disease patients in Israel [Elektronische Daten] [Zeev Meiner, Esther Kahana, Fanny Baitcher, Amos Korczyn, Joab Chapman, Oren Cohen, Ron Milo, Judith Aharon-Perez, Oded Abramsky, Ruth Gabizon, Hanna Rosenmann] One of the largest clusters of genetic Creutzfeldt-Jakob disease (gCJD) is found among Jews of Libyan origin in Israel and is linked to the E200K mutation in PRNP (gCJDE200K). The aim of this study was to compare the levels of cerebrospinal fluid (CSF) biomarkers, Tau and 14-3-3 proteins, between gCJDE200K patients, sporadic CJD (sCJD) patients and non-CJD controls in Israel between the years 1996-2006. The levels of Tau and 14-3-3 proteins in CSF were measured by ELISA and immunoblotting, respectively. CSF Tau levels were similar in gCJDE200K and sCJD, both were significantly higher than in controls [1,107±470pg/ml [33/46 (72%)] of the cases >1,000pg/ml, 1,280±580pg/ml [25/30 (83.3%)], and 354±338pg/ml [17/243 (6.9%)], respectively, p<0.001]. 14-3-3 was detected in CSF of 41/53 (77%) of each gCJDE200K and sCJD patients tested, but only in 70/417 (16.8%) of controls (p<0.001). An inverse correlation was found between disease duration and Tau levels in both gCJDE200K and sCJD (r=−0.464 and r=−0.284). No difference was found in Tau or 14-3-3 between the various codon 129 genotypes. We conclude that CSF biomarkers, Tau and 14-3-3, may be used in the diagnosis in both patients' populations, presenting a similar sensitivity yet Tau assay having higher specificity. Springer-Verlag, 2010 Tau nationallicence 14-3-3 protein nationallicence Cerebrospinal fluid nationallicence Creutzfeldt-Jakob disease nationallicence Libyan Jews nationallicence Meiner Zeev Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut Kahana Esther Department of Neurology, Barzilai Medical Center, Ashkelon, Israel aut Baitcher Fanny Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut Korczyn Amos Sieratzky Chair of Neurology, Tel Aviv University, Tel Aviv, Israel aut Chapman Joab Department of Neurology, Sheba Medical Center, Tel Aviv, Israel aut Cohen Oren Department of Neurology, Sheba Medical Center, Tel Aviv, Israel aut Milo Ron Department of Neurology, Barzilai Medical Center, Ashkelon, Israel aut Aharon-Perez Judith Department of Neurology, Rambam Medical Center, Haifa, Israel aut Abramsky Oded Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut Gabizon Ruth Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut Rosenmann Hanna Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut Journal of Neurology Springer-Verlag 258/2(2011-02-01), 255-262 0340-5354 258:2<255 2011 258 415 https://doi.org/10.1007/s00415-010-5738-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00415-010-5738-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meiner Zeev Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut NATIONALLICENCE 700 1- Kahana Esther Department of Neurology, Barzilai Medical Center, Ashkelon, Israel aut NATIONALLICENCE 700 1- Baitcher Fanny Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut NATIONALLICENCE 700 1- Korczyn Amos Sieratzky Chair of Neurology, Tel Aviv University, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Chapman Joab Department of Neurology, Sheba Medical Center, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Cohen Oren Department of Neurology, Sheba Medical Center, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Milo Ron Department of Neurology, Barzilai Medical Center, Ashkelon, Israel aut NATIONALLICENCE 700 1- Aharon-Perez Judith Department of Neurology, Rambam Medical Center, Haifa, Israel aut NATIONALLICENCE 700 1- Abramsky Oded Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut NATIONALLICENCE 700 1- Gabizon Ruth Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut NATIONALLICENCE 700 1- Rosenmann Hanna Departments of Neurology, Hadassah University Hospital, Jerusalem, Israel aut NATIONALLICENCE 773 0- Journal of Neurology Springer-Verlag 258/2(2011-02-01), 255-262 0340-5354 258:2<255 2011 258 415 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer