caa a22 4500 445812648 CHVBK 20180317145216.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00411-010-0341-x doi (NATIONALLICENCE)springer-10.1007/s00411-010-0341-x Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck (SCCHN) cell lines [Elektronische Daten] [Hendrik Wolff, David Rolke, Margret Rave-Fränk, Markus Schirmer, Wolfgang Eicheler, Annegret Doerfler, Andrea Hille, Clemens Hess, Christoph Matthias, Ralph Rödel, Hans Christiansen] The purpose of this work was to analyze chemokine and chemokine receptor expression in untreated and in irradiated squamous cell carcinoma of the head and neck (SCCHN) tumor cell lines, aiming at the establishment of assays to test for the relevance of chemokine and chemokine receptor expression in the response of SCCHN to radiotherapy and radiochemotherapy. Five low passage and 10 established SCCHN lines, as well as two normal cell lines, were irradiated at 2Gy or sham-irradiated, and harvested between 1 and 48h after treatment. For chemokines with CC and CXC structural motifs and their receptors, transcript levels of target and reference genes were quantified relatively by real-time PCR. In addition, CXCL1 and CXCL12 protein expression was analyzed by ELISA. A substantial variation in chemokine and chemokine receptor expression between SCCHN was detected. Practically, all cell lines expressed CCL5 and CCL20, while CCL2 was expressed in normal cells and in some of the tumor cell lines. CXCL1, CXCL2, CXCL3, CXCL10, and CXCL11 were expressed in the vast majority of the cell lines, while the expression of CXCL9 and CXCL12 was restricted to fibroblasts and few tumor cell lines. None of the analyzed cell lines expressed the chemokines CCL3, CCL4, or CCL19. Of the receptors, transcript expression of CCR1, CCR2, CCR3, CCR5, CCR7, CCXR2, and CCXR3 was not detected, and CCR6, CXCR1, and CXCR4 expression was restricted to few tumor cells. Radiation caused up- and down-regulation with respect to chemokine expressions, while for chemokine receptor expressions down-regulations were prevailing. CXCL1 and CXCL12 protein expression corresponded well with the mRNA expression. We conclude that the substantial variation in chemokine and chemokine receptor expression between SCCHN offer opportunities for the establishment of assays to test for the relevance of chemokine and chemokine receptor expression in the response of SCCHN to radiotherapy and radiochemotherapy. The Author(s), 2010 Wolff Hendrik Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut Rolke David Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut Rave-Fränk Margret Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut Schirmer Markus Department of Pharmacology, Universitätsmedizin Göttingen, Göttingen, Germany aut Eicheler Wolfgang Department of Radiation Oncology, OncoRay-Center for Radiation Research in Oncology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany aut Doerfler Annegret Department of Radiation Oncology, OncoRay-Center for Radiation Research in Oncology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany aut Hille Andrea Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut Hess Clemens Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut Matthias Christoph Department of Otorhinolaryngology-Head and Neck Surgery, Universitätsmedizin Göttingen, Göttingen, Germany aut Rödel Ralph Department of Otorhinolaryngology-Head and Neck Surgery, Universitätsmedizin Göttingen, Göttingen, Germany aut Christiansen Hans Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 145-154 0301-634X 50:1<145 2011 50 411 https://doi.org/10.1007/s00411-010-0341-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00411-010-0341-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wolff Hendrik Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Rolke David Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Rave-Fränk Margret Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Schirmer Markus Department of Pharmacology, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Eicheler Wolfgang Department of Radiation Oncology, OncoRay-Center for Radiation Research in Oncology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany aut NATIONALLICENCE 700 1- Doerfler Annegret Department of Radiation Oncology, OncoRay-Center for Radiation Research in Oncology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany aut NATIONALLICENCE 700 1- Hille Andrea Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Hess Clemens Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Matthias Christoph Department of Otorhinolaryngology-Head and Neck Surgery, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Rödel Ralph Department of Otorhinolaryngology-Head and Neck Surgery, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Christiansen Hans Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany aut NATIONALLICENCE 773 0- Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 145-154 0301-634X 50:1<145 2011 50 411 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer