caa a22 4500 445812761 CHVBK 20180317145217.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00411-010-0327-8 doi (NATIONALLICENCE)springer-10.1007/s00411-010-0327-8 Headspace measurements of irradiated in vitro cultured cells using PTR-MS [Elektronische Daten] [C. Brunner, W. Szymczak, W. Li, C. Hoeschen, S. Mörtl, F. Eckardt-Schupp, U. Oeh] A pilot study was performed to evaluate a new concept for a radiation biodosimetry method. Proton transfer reaction-mass spectrometry (PTR-MS) was used to find out whether radiation induces changes in the composition of volatile organic compounds (VOCs) in the headspace of in vitro cultured cells. Two different cell lines, retinal pigment epithelium cells hTERT-RPE1 and lung epithelium cells A-549, were irradiated with gamma radiation at doses of 4Gy and 8Gy. For measuring the cell-specific effects, the VOC concentrations in the headspace of flasks containing cells plus medium, as well as of flasks containing pure medium were analyzed for changes before and after irradiation. No significant radiation-induced alterations in VOC concentrations in the headspace could be observed after irradiation. Springer-Verlag, 2010 Brunner C. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut Szymczak W. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut Li W. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut Hoeschen C. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut Mörtl S. Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut Eckardt-Schupp F. Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut Oeh U. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 209-217 0301-634X 50:1<209 2011 50 411 https://doi.org/10.1007/s00411-010-0327-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00411-010-0327-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brunner C. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Szymczak W. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Li W. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Hoeschen C. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Mörtl S. Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Eckardt-Schupp F. Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Oeh U. Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 773 0- Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 209-217 0301-634X 50:1<209 2011 50 411 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer