caa a22 4500 445812788 CHVBK 20180317145217.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00411-010-0342-9 doi (NATIONALLICENCE)springer-10.1007/s00411-010-0342-9 Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA.hy926 [Elektronische Daten] [Franka Pluder, Zarko Barjaktarovic, Omid Azimzadeh, Simone Mörtl, Anne Krämer, Sylvia Steininger, Hakan Sarioglu, Dariusz Leszczynski, Reetta Nylund, Arvi Hakanen, Arundhathi Sriharshan, Michael Atkinson, Soile Tapio] High doses of ionising radiation damage the heart by an as yet unknown mechanism. A concern for radiological protection is the recent epidemiological data indicating that doses as low as 100-500mGy may induce cardiac damage. The aim of this study was to identify potential molecular targets and/or mechanisms involved in the pathogenesis of low-dose radiation-induced cardiovascular disease. The vascular endothelium plays a pivotal role in the regulation of cardiac function and is therefore a potential target tissue. We report here that low-dose radiation induced rapid and time-dependent changes in the cytoplasmic proteome of the human endothelial cell line EA.hy926. The proteomes were investigated at 4 and 24h after irradiation at two different dose rates (Co-60 gamma ray total dose 200mGy; 20mGy/min and 190mGy/min) using 2D-DIGE technology. Differentially expressed proteins were identified, after in-gel trypsin digestion, by MALDI-TOF/TOF tandem mass spectrometry, and peptide mass fingerprint analyses. We identified 15 significantly differentially expressed proteins, of which 10 were up-regulated and 5 down-regulated, with more than±1.5-fold difference compared with unexposed cells. Pathways influenced by the low-dose exposures included the Ran and RhoA pathways, fatty acid metabolism and stress response. Springer-Verlag, 2010 Pluder Franka Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Barjaktarovic Zarko Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Azimzadeh Omid Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Mörtl Simone Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Krämer Anne Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Steininger Sylvia Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Sarioglu Hakan Department of Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany aut Leszczynski Dariusz STUK—Radiation and Nuclear Safety Authority, Helsinki, Finland aut Nylund Reetta STUK—Radiation and Nuclear Safety Authority, Helsinki, Finland aut Hakanen Arvi STUK—Radiation and Nuclear Safety Authority, Helsinki, Finland aut Sriharshan Arundhathi Division of Radiation Cytogenetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany aut Atkinson Michael Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Tapio Soile Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 155-166 0301-634X 50:1<155 2011 50 411 https://doi.org/10.1007/s00411-010-0342-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00411-010-0342-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pluder Franka Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Barjaktarovic Zarko Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Azimzadeh Omid Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Mörtl Simone Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Krämer Anne Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Steininger Sylvia Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Sarioglu Hakan Department of Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany aut NATIONALLICENCE 700 1- Leszczynski Dariusz STUK—Radiation and Nuclear Safety Authority, Helsinki, Finland aut NATIONALLICENCE 700 1- Nylund Reetta STUK—Radiation and Nuclear Safety Authority, Helsinki, Finland aut NATIONALLICENCE 700 1- Hakanen Arvi STUK—Radiation and Nuclear Safety Authority, Helsinki, Finland aut NATIONALLICENCE 700 1- Sriharshan Arundhathi Division of Radiation Cytogenetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany aut NATIONALLICENCE 700 1- Atkinson Michael Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 700 1- Tapio Soile Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany aut NATIONALLICENCE 773 0- Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 155-166 0301-634X 50:1<155 2011 50 411 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer