caa a22 4500 445812818 CHVBK 20180317145217.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00411-010-0329-6 doi (NATIONALLICENCE)springer-10.1007/s00411-010-0329-6 Alteration of mtDNA copy number, mitochondrial gene expression and extracellular DNA content in mice after irradiation at lethal dose [Elektronische Daten] [Edward Evdokimovsky, Tatjana Ushakova, Andrej Kudriavtcev, Ajub. Gaziev] High steady-state transcriptional activity is essential for normal mitochondrial function. The requisite transcription rate is satisfied in part by high copy number of mitochondrial DNA (mtDNA). In the present study, we analyze mtDNA copy number by real-time PCR in nucleated blood cells from control mice and mice exposed to 1- or 10-Gy X-radiation. Transcription of the oxidative phosphorylation-associated genes cytb, atp6, nd4, nd2 and d-loop region was monitored in these nucleated blood cells similarly by real-time PCR. We observed a 50% decrease in the ratio of mitochondrial to nuclear DNA (mtDNA/nDNA) in blood cells, while the mtDNA/nDNA ratio in serum increased. After a lethal 10-Gy dose of X-irradiation, we observed an 80% decrease in the number of circulating lymphocytes. In response to a 10-Gy radiation dose, we observed transiently increased mtDNA/nDNA ratio and transcription within the initial 5h post-treatment. At 24-72h, the mtDNA/nDNA ratio in surviving cells was reduced to the level observed in blood cells irradiated with 1Gy. We observed a decrease in the serum mtDNA/nDNA ratio due to an increase in nDNA content rather than a decrease in mtDNA. Taken together, results presented herein suggest that the mtDNA/nDNA ratio may be of clinical value potentially as a diagnostic tool, particularly in oncology patients undergoing radiation therapy. Springer-Verlag, 2010 Evdokimovsky Edward Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut Ushakova Tatjana Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut Kudriavtcev Andrej Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut Gaziev Ajub Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 181-188 0301-634X 50:1<181 2011 50 411 https://doi.org/10.1007/s00411-010-0329-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00411-010-0329-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Evdokimovsky Edward Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut NATIONALLICENCE 700 1- Ushakova Tatjana Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut NATIONALLICENCE 700 1- Kudriavtcev Andrej Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut NATIONALLICENCE 700 1- Gaziev Ajub Laboratory of Radiation and Molecular Biology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Instituskaya St., 142290, Pushchino, Russia aut NATIONALLICENCE 773 0- Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 181-188 0301-634X 50:1<181 2011 50 411 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer