caa a22 4500 445812826 CHVBK 20180317145217.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00411-010-0348-3 doi (NATIONALLICENCE)springer-10.1007/s00411-010-0348-3 Frozen human cells can record radiation damage accumulated during space flight: mutation induction and radioadaptation [Elektronische Daten] [Fumio Yatagai, Masamitsu Honma, Akihisa Takahashi, Katsunori Omori, Hiromi Suzuki, Toru Shimazu, Masaya Seki, Toko Hashizume, Akiko Ukai, Kaoru Sugasawa, Tomoko Abe, Naoshi Dohmae, Shuichi Enomoto, Takeo Ohnishi, Alasdair Gordon, Noriaki Ishioka] To estimate the space-radiation effects separately from other space-environmental effects such as microgravity, frozen human lymphoblastoid TK6 cells were sent to the "Kibo” module of the International Space Station (ISS), preserved under frozen condition during the mission and finally recovered to Earth (after a total of 134days flight, 72mSv). Biological assays were performed on the cells recovered to Earth. We observed a tendency of increase (2.3-fold) in thymidine kinase deficient (TK−) mutations over the ground control. Loss of heterozygosity (LOH) analysis on the mutants also demonstrated a tendency of increase in proportion of the large deletion (beyond the TK locus) events, 6/41 in the in-flight samples and 1/17 in the ground control. Furthermore, in-flight samples exhibited 48% of the ground-control level in TK− mutation frequency upon exposure to a subsequent 2Gy dose of X-rays, suggesting a tendency of radioadaptation when compared with the ground-control samples. The tendency of radioadaptation was also supported by the post-flight assays on DNA double-strand break repair: a 1.8- and 1.7-fold higher efficiency of in-flight samples compared to ground control via non-homologous end-joining and homologous recombination, respectively. These observations suggest that this system can be used as a biodosimeter, because DNA damage generated by space radiation is considered to be accumulated in the cells preserved frozen during the mission, Furthermore, this system is also suggested to be applicable for evaluating various cellular responses to low-dose space radiation, providing a better understanding of biological space-radiation effects as well as estimation of health influences of future space explores. Springer-Verlag, 2010 Yatagai Fumio The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut Honma Masamitsu Division of Genetics and Mutagenesis, National Institute of Health Sciences, 158-8501, Tokyo, Japan aut Takahashi Akihisa Biology Lab, Nara Medical University, 634-8521, Nara, Japan aut Omori Katsunori Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 305-8505, Ibaraki, Japan aut Suzuki Hiromi Japan Space Forum, 104-0004, Tokyo, Japan aut Shimazu Toru Japan Space Forum, 104-0004, Tokyo, Japan aut Seki Masaya Advanced Engineering Services, 103-0028, Tokyo, Japan aut Hashizume Toko Advanced Engineering Services, 103-0028, Tokyo, Japan aut Ukai Akiko Division of Genetics and Mutagenesis, National Institute of Health Sciences, 158-8501, Tokyo, Japan aut Sugasawa Kaoru Biosignal Research Center, Kobe University, 657-8501, Hyogo, Japan aut Abe Tomoko The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut Dohmae Naoshi The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut Enomoto Shuichi The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut Ohnishi Takeo Biology Lab, Nara Medical University, 634-8521, Nara, Japan aut Gordon Alasdair Baylor College of Medicine, 77030, Houston, TX, USA aut Ishioka Noriaki Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 305-8505, Ibaraki, Japan aut Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 125-134 0301-634X 50:1<125 2011 50 411 https://doi.org/10.1007/s00411-010-0348-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00411-010-0348-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yatagai Fumio The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Honma Masamitsu Division of Genetics and Mutagenesis, National Institute of Health Sciences, 158-8501, Tokyo, Japan aut NATIONALLICENCE 700 1- Takahashi Akihisa Biology Lab, Nara Medical University, 634-8521, Nara, Japan aut NATIONALLICENCE 700 1- Omori Katsunori Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 305-8505, Ibaraki, Japan aut NATIONALLICENCE 700 1- Suzuki Hiromi Japan Space Forum, 104-0004, Tokyo, Japan aut NATIONALLICENCE 700 1- Shimazu Toru Japan Space Forum, 104-0004, Tokyo, Japan aut NATIONALLICENCE 700 1- Seki Masaya Advanced Engineering Services, 103-0028, Tokyo, Japan aut NATIONALLICENCE 700 1- Hashizume Toko Advanced Engineering Services, 103-0028, Tokyo, Japan aut NATIONALLICENCE 700 1- Ukai Akiko Division of Genetics and Mutagenesis, National Institute of Health Sciences, 158-8501, Tokyo, Japan aut NATIONALLICENCE 700 1- Sugasawa Kaoru Biosignal Research Center, Kobe University, 657-8501, Hyogo, Japan aut NATIONALLICENCE 700 1- Abe Tomoko The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Dohmae Naoshi The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Enomoto Shuichi The Institute of Physical and Chemical Research (RIKEN), 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Ohnishi Takeo Biology Lab, Nara Medical University, 634-8521, Nara, Japan aut NATIONALLICENCE 700 1- Gordon Alasdair Baylor College of Medicine, 77030, Houston, TX, USA aut NATIONALLICENCE 700 1- Ishioka Noriaki Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 305-8505, Ibaraki, Japan aut NATIONALLICENCE 773 0- Radiation and Environmental Biophysics Springer-Verlag 50/1(2011-03-01), 125-134 0301-634X 50:1<125 2011 50 411 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer