caa a22 4500 445813148 CHVBK 20180317145218.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00411-011-0352-2 doi (NATIONALLICENCE)springer-10.1007/s00411-011-0352-2 Protective effects of selenocystine against γ-radiation-induced genotoxicity in Swiss albino mice [Elektronische Daten] [Amit Kunwar, S. Jayakumar, H. Bhilwade, P. Bag, H. Bhatt, R. Chaubey, K. Priyadarsini] Selenocystine (CysSeSeCys), a diselenide aminoacid exhibiting glutathione peroxidase-like activity and selective antitumor effects, was examined for in vivo antigenotoxic and antioxidant activity in Swiss albino mice after exposure to a sublethal dose (5Gy) of γ-radiation. For this, CysSeSeCys was administered intraperitoneally (i.p.) to mice at a dosage of 0.5mg/kg body weight for 5 consecutive days prior to whole-body γ-irradiation. When examined in the hepatic tissue, CysSeSeCys administration reduced the DNA damage at 30min after radiation exposure by increasing the rate of DNA repair. Since antigenotoxic agents could alter the expression of genes involved in cell cycle arrest and DNA repair, the transcriptional changes in p53, p21 and GADD45α were monitored in the hepatic tissue by real-time PCR. The results show that CysSeSeCys alone causes moderate induction of these three genes. However, CysSeSeCys pretreatment resulted in a suppression of radiation-induced enhancement of p21 and GADD45α expression, but did not affect p53 expression. Further analysis of radiation-induced oxidative stress markers in the same tissue indicated that CysSeSeCys significantly inhibits lipid peroxidation and prevents the depletion of antioxidant enzymes and glutathione (GSH) levels. Additionally, it also prevents radiation-induced DNA damage in other radiation sensitive cellular systems like peripheral leukocytes and bone marrow, which was evident by a decrease in comet parameters and micronucleated polychromatic erythrocytes (mn-PCEs) frequency, respectively. Based on these observations, it is concluded that CysSeSeCys exhibits antigenotoxic effects, reduces radiation-induced oxidative stress, and is a promising candidate for future exploration as a radioprotector. Springer-Verlag, 2011 Kunwar Amit Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Jayakumar S. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Bhilwade H. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Bag P. Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Bhatt H. Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Chaubey R. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Priyadarsini K. Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Radiation and Environmental Biophysics Springer-Verlag 50/2(2011-05-01), 271-280 0301-634X 50:2<271 2011 50 411 https://doi.org/10.1007/s00411-011-0352-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00411-011-0352-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kunwar Amit Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Jayakumar S. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Bhilwade H. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Bag P. Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Bhatt H. Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Chaubey R. Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Priyadarsini K. Radiation & Photochemistry Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 773 0- Radiation and Environmental Biophysics Springer-Verlag 50/2(2011-05-01), 271-280 0301-634X 50:2<271 2011 50 411 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer