caa a22 4500 445813202 CHVBK 20180317145218.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00411-011-0353-1 doi (NATIONALLICENCE)springer-10.1007/s00411-011-0353-1 Dose rate effect on micronuclei induction in human blood lymphocytes exposed to single pulse and multiple pulses of electrons [Elektronische Daten] [Santhosh Acharya, N. Bhat, Praveen Joseph, Ganesh Sanjeev, B. Sreedevi, Y. Narayana] The effects of single pulses and multiple pulses of 7 MV electrons on micronuclei (MN) induction in cytokinesis-blocked human peripheral blood lymphocytes (PBLs) were investigated over a wide range of dose rates per pulse (instantaneous dose rate). PBLs were exposed to graded doses of 2, 3, 4, 6, and 8Gy of single electron pulses of varying pulse widths at different dose rates per pulse, ranging from 1×106 Gys−1 to 3.2×108 Gys−1. Different dose rates per pulse were achieved by changing the dose per electron pulse by adjusting the beam current and pulse width. MN yields per unit absorbed dose after irradiation with single electron pulses were compared with those of multiple pulses of electrons. A significant decrease in the MN yield with increasing dose rates per pulse was observed, when dose was delivered by a single electron pulse. However, no reduction in the MN yield was observed when dose was delivered by multiple pulses of electrons. The decrease in the yield at high dose rates per pulse suggests possible radical recombination, which leads to decreased biological damage. Cellular response to the presence of very large numbers of chromosomal breaks may also alter the damage. Springer-Verlag, 2011 Acharya Santhosh Department of Engineering Physics, Alva's Institute of Engineering and Technology, 574225, Moodbidri, India aut Bhat N. Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), 400085, Mumbai, India aut Joseph Praveen Department of Studies in Physics, Microtron Centre, Mangalore University, 574199, Mangalagangotri, India aut Sanjeev Ganesh Department of Studies in Physics, Microtron Centre, Mangalore University, 574199, Mangalagangotri, India aut Sreedevi B. Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), 400085, Mumbai, India aut Narayana Y. Department of Studies in Physics, Microtron Centre, Mangalore University, 574199, Mangalagangotri, India aut Radiation and Environmental Biophysics Springer-Verlag 50/2(2011-05-01), 253-263 0301-634X 50:2<253 2011 50 411 https://doi.org/10.1007/s00411-011-0353-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00411-011-0353-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Acharya Santhosh Department of Engineering Physics, Alva's Institute of Engineering and Technology, 574225, Moodbidri, India aut NATIONALLICENCE 700 1- Bhat N. Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), 400085, Mumbai, India aut NATIONALLICENCE 700 1- Joseph Praveen Department of Studies in Physics, Microtron Centre, Mangalore University, 574199, Mangalagangotri, India aut NATIONALLICENCE 700 1- Sanjeev Ganesh Department of Studies in Physics, Microtron Centre, Mangalore University, 574199, Mangalagangotri, India aut NATIONALLICENCE 700 1- Sreedevi B. Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), 400085, Mumbai, India aut NATIONALLICENCE 700 1- Narayana Y. Department of Studies in Physics, Microtron Centre, Mangalore University, 574199, Mangalagangotri, India aut NATIONALLICENCE 773 0- Radiation and Environmental Biophysics Springer-Verlag 50/2(2011-05-01), 253-263 0301-634X 50:2<253 2011 50 411 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer