caa a22 4500 445816465 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10815-010-9481-9 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9481-9 The cumulus cell gene expression profile of oocytes with different nuclear maturity and potential for blastocyst formation [Elektronische Daten] [Tom Adriaenssens, Ingrid Segers, Sandra Wathlet, Johan Smitz] Purpose: Gene expression in human ART cumulus cell (CC) has been related to oocyte maturity and competence but requires further validation. Expression dynamics were investigated in CC of oocytes at different maturational stages and with different developmental competence in a standard in vivo mouse superovulation model. Methods: Quantitative PCR analysis of Has2, Vcan, Sdc4, Alcam, Grem1, Ptgs1 and Ptgs2 in CC collected at regular time intervals from 0 to 24h post hCG injection. Results: Three expression patterns were observed each with strong regulation (4-230× differences). Immediately prior to ovulation CC of GVBD oocytes have 5× less Sdc4 and Ptgs1 and 5× more Ptgs2 when compared to the CC of freshly ovulated PB oocytes. When compared to the latter, the post-ovulatory aged PB oocytes had a 2× reduced blastocyst forming capacity and their CC expressed 2× more Sdc4 and 6× less Alcam. Conclusions: Morphologically identical cumulus oocyte complexes with different developmental competence can be differentiated by CC gene expression. Springer Science+Business Media, LLC, 2010 Cumulus cells nationallicence Gene expression nationallicence Oocyte maturation nationallicence Oocyte quality nationallicence Adriaenssens Tom Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut Segers Ingrid Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut Wathlet Sandra Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut Smitz Johan Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 31-40 1058-0468 28:1<31 2011 28 10815 https://doi.org/10.1007/s10815-010-9481-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9481-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adriaenssens Tom Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Segers Ingrid Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Wathlet Sandra Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Smitz Johan Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B 1090, Brussels, Belgium aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 31-40 1058-0468 28:1<31 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer