caa a22 4500 44581649X CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10815-010-9477-5 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9477-5 Whole sheep ovary cryopreservation: evaluation of a slow freezing protocol with dimethylsulphoxide [Elektronische Daten] [Milan Milenkovic, Ann Wallin, Manda Ghahremani, Mats Brännström] Purpose: To evaluate a slow freezing method for whole ovary cryopreservation by evaluating effects of added cryoprotectant. Methods: Sheep ovaries were isolated during surgery, flushed with either Ringer-Acetate or dimethylsulphoxide and cryopreserved by slow freezing. After rapid thawing, viability was assessed by ovarian in vitro perfusion, cell culture, histology and fluorescent live-dead assay. Results: Production of cyclic AMP and progesterone was slightly higher in the dimethylsulphoxide group. Cultured ovarian cells from dimethylsulphoxide-preserved ovaries secreted larger amounts of progesterone than cells from Ringer-Acetate preserved. Light microscopy of ovarian biopsies obtained after perfusion, revealed well-preserved tissue in the dimethysulphoxide group but not in the Ringer-Acetate group. The density of small follicles and ovarian cell viability were higher in dimethysulphoxide ovaries compared to Ringer-Acetate ovaries. Conclusions: Equilibrium with its protective effect can be achieved by slow freezing protocol, with an additional protective effect by the presence of dimethylsulphoxide. Springer Science+Business Media, LLC, 2010 Cryopreservation nationallicence Cyclic AMP nationallicence Dimethylsulphoxide nationallicence Ovary nationallicence Perfusion nationallicence Milenkovic Milan Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Göteborg, Sweden aut Wallin Ann Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Göteborg, Sweden aut Ghahremani Manda Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada aut Brännström Mats Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Göteborg, Sweden aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 7-14 1058-0468 28:1<7 2011 28 10815 https://doi.org/10.1007/s10815-010-9477-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9477-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Milenkovic Milan Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Göteborg, Sweden aut NATIONALLICENCE 700 1- Wallin Ann Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Göteborg, Sweden aut NATIONALLICENCE 700 1- Ghahremani Manda Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Brännström Mats Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Göteborg, Sweden aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 7-14 1058-0468 28:1<7 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer