caa a22 4500 44581652X CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10815-010-9482-8 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9482-8 Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia [Elektronische Daten] [Sonia Brahem, Meriem Mehdi, Hatem Elghezal, Ali Saad] Purpose: To evaluate levels of DNA fragmentation and chromosomal abnormalities in ejaculated sperm of males with isolated teratozoospermia and to determine if specific sperm morphological types occur simultaneously with these nuclear defects. Methods: Sperm obtained from isolated teratozoospermic men (n = 70) and fertile men (n = 30) were analysed using fluorescence in situ hybridization and TUNEL assay. Results: Teratozoospermic men, compared to fertile men, showed significantly higher rates of sex chromosomes disomy, and diploidy. Significant correlations were found between amorphous head, microcephalic head, short tail, and sex chromosomes disomy. Level of sperm DNA fragmentation was significantly higher in teratozoospermic men than in controls and positively correlated to the incidence of macrocephalic heads, amorphous heads, and short flagella. Conclusions: Patients with isolated teratozoospermia have increased levels of DNA fragmentation and chromosomal aneuploidy. Some specific morphological abnormalities were shown to be predictive of chromosomal abnormalities and DNA alteration. Springer Science+Business Media, LLC, 2010 Aneuploidy nationallicence DNA fragmentation nationallicence Infertility nationallicence Teratozoospermia nationallicence Brahem Sonia Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Mehdi Meriem Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Elghezal Hatem Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Saad Ali Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 41-48 1058-0468 28:1<41 2011 28 10815 https://doi.org/10.1007/s10815-010-9482-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9482-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brahem Sonia Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Mehdi Meriem Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Elghezal Hatem Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Saad Ali Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 41-48 1058-0468 28:1<41 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer