caa a22 4500 445816546 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10815-010-9480-x doi (NATIONALLICENCE)springer-10.1007/s10815-010-9480-x Effects of L- and T-type Ca2+ channel blockers on spermatogenesis and steroidogenesis in the prepubertal mouse testis [Elektronische Daten] [Jae Lee, Hak Ahn, Sang Lee, Myung Gye, Churl Min] Purpose: To assess the involvement of L-type and T-type Ca2+ channel blockers in inducing male infertility. Methods: Prepubertal male mice were fed Ca2+ channel blockers nifedipine and ethosuximide for 20days at dosages below maximum tolerated dose (MTD) and assayed for gross morphological changes in the testis such as body weight, testis size and weight. Sperm and Leydig cell counting were conducted concomitantly with serum testosterone level measurement by radioimmunoassay (RIA) and StAR protein mRNA measurement by reverse transcription and polymerase chain reaction (RT-PCR). Results: A chronic exposure to nifedipine or ethosuximide caused a significant reduction in body weight, testis size/weight and sperm production in a dose-dependent fashion associated with a spermatogenic arrest largely at the elongating spermatid stage. The number of Leydig cells, the serum testosterone level but not the luteinizing hormone level, and the content of StAR protein mRNA were also drastically reduced relative to the controls. Conclusions: Both T- and L-type Ca2+ channel blockers play an adverse role in normal spermatogenesis and steroidogenesis partly by blocking postmeiotic germ cell maturation and/or by abrogating StAR protein expression, contributing to male sterility. Therefore, any therapeutic application of Ca2+ channel blockers must be used with caution due to its potential adverse side effects on male infertility. Springer Science+Business Media, LLC, 2010 Ca2+ channel blockers nationallicence Spermatogenesis nationallicence Steroidogenesis nationallicence Mouse prepubertal testis nationallicence Lee Jae Institute of Tissue Regeneration Engineering, Dankook University, 330-714, Cheonan, South Korea aut Ahn Hak Department of Molecular Sciences and Technology, Ajou University, 443-749, Suwon, South Korea aut Lee Sang Department of Animal Science, Sahmyook College, 139-742, Seoul, South Korea aut Gye Myung Department of Life Science, Hanyang University, 133-791, Seoul, South Korea aut Min Churl Department of Biological Sciences, Ajou University, 443-749, Suwon, South Korea aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 23-30 1058-0468 28:1<23 2011 28 10815 https://doi.org/10.1007/s10815-010-9480-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9480-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Jae Institute of Tissue Regeneration Engineering, Dankook University, 330-714, Cheonan, South Korea aut NATIONALLICENCE 700 1- Ahn Hak Department of Molecular Sciences and Technology, Ajou University, 443-749, Suwon, South Korea aut NATIONALLICENCE 700 1- Lee Sang Department of Animal Science, Sahmyook College, 139-742, Seoul, South Korea aut NATIONALLICENCE 700 1- Gye Myung Department of Life Science, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Min Churl Department of Biological Sciences, Ajou University, 443-749, Suwon, South Korea aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 23-30 1058-0468 28:1<23 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer