caa a22 4500 445816562 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10815-010-9487-3 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9487-3 Successful live birth from vitrified oocytes after 5 years of cryopreservation [Elektronische Daten] [Thomas Kim, Seung Hong] Objective: To report a birth of a healthy boy after long-term cryopreservation of oocytes by vitrification. Design: Clinical application. Setting: IVF Center. Patient: A 17year-old female with secondary pulmonary hypertension caused by transposition of great vessels visited our center in 2002, and she wished oocytes cryopreservation to avoid possible sterility after the following category X medication treatment. Intervention(s): Vitrified oocytes on Electron Microscope (EM) grids were warmed after 5years of storage. Surviving MII oocytes were microinjected for fertilization and two embryos were transferred into a gestational carrier day 5 after microinjection. Main Outcome Measure(s): Survival, fertilization, cleavage, clinical pregnancy and delivery. Result(s): Eleven out of fourteen oocytes (78.6%) survived warming. Eight Metaphase II (MII) oocytes and 3 in vitro matured oocytes were microinjected; all 11 oocytes (100%) fertilized and 2 embryos were transferred on day 5. A healthy baby boy weighing 3,600g was delivered at 38weeks of gestation. Live-birth rates per warmed oocyte and per injected oocyte were 7.1% and 9.1% respectively. Conclusion(s): Cryopreservation after vitrification with EM grids maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth. Springer Science+Business Media, LLC, 2010 Human oocyte cryopreservation nationallicence Vitrification nationallicence Electron microscope grid nationallicence Ethylene glycol nationallicence Clinical outcome nationallicence Kim Thomas Reproductive Medicine Associates of New Jersey, 07960, Morristown, NJ, USA aut Hong Seung CHA Fertility Center, 5455 Wilshire Boulevard, Suite 1904, 90036, Los Angeles, CA, USA aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 73-76 1058-0468 28:1<73 2011 28 10815 https://doi.org/10.1007/s10815-010-9487-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9487-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Thomas Reproductive Medicine Associates of New Jersey, 07960, Morristown, NJ, USA aut NATIONALLICENCE 700 1- Hong Seung CHA Fertility Center, 5455 Wilshire Boulevard, Suite 1904, 90036, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/1(2011-01-01), 73-76 1058-0468 28:1<73 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer