caa a22 4500 445816597 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10815-011-9555-3 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9555-3 Correlation of oocyte morphometry parameters with woman's age [Elektronische Daten] [Claudia Valeri, Sebastiana Pappalardo, Massimo De Felici, Claudio Manna] Purpose: Aim of this study was to evaluate morphometric parameters of metaphase II oocytes, including cytoplasm diameter (CD), zona pellucida thickness (ZPT) and width of the perivitelline space (PS), in relation with zona pellucida birefringence, spindle presence and age of the woman. Methods: Oocytes were classified into groups according to zona birefringence (low or high zona birefringence, LZB and HZB, respectively) and presence or absence of a visible spindle (SP and aSP, respectively). Results: HZB oocytes showed a thicker zona (17.7 ± 0.3μm) than LZB oocytes (16.7 ± 0.3μm, p < 0.01). Moreover, PS was narrower in HZB and SP oocytes than in LZB (p < 0,001) and aSP (p < 0,05) oocytes. Finally, we found that CD and ZPT linearly decrease with age of the woman (CD r = 0.028: p < 0.01; ZPT r = 0.050: p < 0.0001). Conclusion: Our results evidence an association in human oocytes between zona pellucida and spindle birefringence and defined morphometric parameters and a decrease of oocyte size and ZPT as a function of women's age. Springer Science+Business Media, LLC, 2011 Birefringence nationallicence Morphometry nationallicence Oocyte aging nationallicence Zona pellucida nationallicence Valeri Claudia Genesis Centre for Reproductive Medicine, via Velletri 7, 00198, Rome, Italy aut Pappalardo Sebastiana Genesis Centre for Reproductive Medicine, via Velletri 7, 00198, Rome, Italy aut De Felici Massimo Department of Public Health and Cell Biology, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy aut Manna Claudio Genesis Centre for Reproductive Medicine, via Velletri 7, 00198, Rome, Italy aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 545-552 1058-0468 28:6<545 2011 28 10815 https://doi.org/10.1007/s10815-011-9555-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9555-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valeri Claudia Genesis Centre for Reproductive Medicine, via Velletri 7, 00198, Rome, Italy aut NATIONALLICENCE 700 1- Pappalardo Sebastiana Genesis Centre for Reproductive Medicine, via Velletri 7, 00198, Rome, Italy aut NATIONALLICENCE 700 1- De Felici Massimo Department of Public Health and Cell Biology, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Manna Claudio Genesis Centre for Reproductive Medicine, via Velletri 7, 00198, Rome, Italy aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 545-552 1058-0468 28:6<545 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer