caa a22 4500 445816627 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10815-011-9579-8 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9579-8 Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system [Elektronische Daten] [Leila Tahaei, Hussein Eimani, Poopak Yazdi, Bita Ebrahimi, Rouhollah Fathi] Purpose: Evaluation of the all-trans retinoic acid (t-RA) effects on in vitro maturation (IVM) and in vitro fertilization (IVF) of immature mouse oocytes in the presence and absence of granulosa cell monolayer. Methods: Denuded oocytes isolated from mice ovaries and matured in IVM medium alone (Control I), IVM medium in the presence of granulosa cells (Control II), IVM medium with t-RA (Experimental I) and IVM medium simultaneously with t-RA and granulosa cells (Experimental II). After 24h, matured oocytes were fertilized in T6 medium and their development was followed until the blastocyst stage. Metaphase II oocytes ploidy were evaluated by chromosome counting. Results: The t-RA group compared to the control groups showed no obvious abnormalities. Additionally maturation and embryo development rates significantly increased in the t-RA treated granulosa cell co-culture system. Conclusions: In conclusion, association of t-RA with granulosa cell co-culture during in vitro maturation increases meiosis resumption, formation of metaphase II oocytes, as well as 2-cell and blastocyst stage embryos. Springer Science+Business Media, LLC, 2011 In vitro maturation nationallicence Granulosa cells nationallicence Immature oocytes nationallicence Retinoic acid co- culture nationallicence Tahaei Leila Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut Eimani Hussein Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut Yazdi Poopak Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut Ebrahimi Bita Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut Fathi Rouhollah Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 553-558 1058-0468 28:6<553 2011 28 10815 https://doi.org/10.1007/s10815-011-9579-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9579-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tahaei Leila Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut NATIONALLICENCE 700 1- Eimani Hussein Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut NATIONALLICENCE 700 1- Yazdi Poopak Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut NATIONALLICENCE 700 1- Ebrahimi Bita Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut NATIONALLICENCE 700 1- Fathi Rouhollah Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, P. O. Box: 166565-9711, Tehran, Iran aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 553-558 1058-0468 28:6<553 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer