caa a22 4500 44581666X CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10815-011-9594-9 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9594-9 Molecular characterization of the human ovulatory cascade—Lesson from the IVF/IVM model [Elektronische Daten] [Gil Yerushalmi, Ettie Maman, Yuval Yung, Alon Kedem, Ariel Hourvitz] Aims: Ovarian follicular development and ovulation in mammals is a complex and highly regulated process. Most advances in the understanding of the ovulatory process have come from animal models. However, translational research in humans is of crucial importance for improving fertility treatment and control. Methods: IVM/IVF procedures allow us to obtain follicular fluid and granulosa cells (GC) from follicles in different developmental stages with and without hCG priming. Results: Using the cells and fluids obtained in IVM/IVF procedures allowed us to characterize human ovulatory gene expression during antral folliculogenesis and ovulation, examine gene expression in luteinized and non-luteinized GC in vivo and in vitro and to use cumulus GC genes as biomarkers for oocyte and embryo maturity and competence. Conclusion: Biological material obtained during IVM/IVF procedures is an important tool to study the human ovulatory cascade and can serve to improve IVM techniques and fertility treatment and control. Springer Science+Business Media, LLC, 2011 IVM nationallicence IVF nationallicence Model nationallicence Ovulation nationallicence Ovulatory genes nationallicence Yerushalmi Gil IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut Maman Ettie IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut Yung Yuval IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut Kedem Alon IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut Hourvitz Ariel IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 509-515 1058-0468 28:6<509 2011 28 10815 https://doi.org/10.1007/s10815-011-9594-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9594-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yerushalmi Gil IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Maman Ettie IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Yung Yuval IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Kedem Alon IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Hourvitz Ariel IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 509-515 1058-0468 28:6<509 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer