caa a22 4500 445816678 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10815-011-9590-0 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9590-0 Differentially expressed micoRNAs in human oocytes [Elektronische Daten] [Yan-Wen Xu, Bin Wang, Chen-Hui Ding, Tao Li, Fang Gu, Canquan Zhou] Purpose: To identify differentially expressed microRNAs (miRNAs) and expression patterns of specific miRNAs during meiosis in human oocytes. Materials and methods: To identify differentially expressed miRNAs, GV oocytes and MII oocytes matured at conventional FSH levels (5.5ng/ml) were analyzed by miRNA microarray. Real-time RT-PCR was used to confirm the changed miRNAs. To validate the dynamic changes of miRNAs from GV to MII stages, oocytes were divided into four groups (#1-4), corresponding to GV oocytes, MI oocytes, MII oocytes matured in conventional FSH level and MII oocytes matured in high FSH level (2,000ng/ml) respectively. Results: Compared with GV oocytes, MII oocytes exhibited up-regulation of 4 miRNAs (hsa-miR-193a-5p, hsa-miR-297, hsa-miR-625 and hsa-miR-602), and down-regulation of 11 miRNAs (hsa-miR-888*, hsa-miR-212, hsa-miR-662, hsa-miR-299-5p, hsa-miR-339-5p, hsa-miR-20a, hsa-miR-486-5p, hsa-miR-141*, hsa-miR-768-5p, hsa-miR-376a and hsa-miR-15a). RT-PCR analysis of hsa-miR-15a and hsa-miR-20a expression revealed concordant dynamic changes in oocytes from group 1 to group 4. Conclusion(s): Specific miRNAs in human oocytes had dynamic changes during meiosis. High-concentration FSH in IVM medium led to reverse effect on the expression of hsa-miR-15a and hsa-miR-20a. Springer Science+Business Media, LLC, 2011 miRNA nationallicence Oocytes nationallicence In vitro maturation nationallicence qRT-PCR nationallicence Xu Yan-Wen Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut Wang Bin Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut Ding Chen-Hui Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut Li Tao Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut Gu Fang Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut Zhou Canquan Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 559-566 1058-0468 28:6<559 2011 28 10815 https://doi.org/10.1007/s10815-011-9590-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9590-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Yan-Wen Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Bin Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut NATIONALLICENCE 700 1- Ding Chen-Hui Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut NATIONALLICENCE 700 1- Li Tao Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut NATIONALLICENCE 700 1- Gu Fang Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut NATIONALLICENCE 700 1- Zhou Canquan Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan Rd 2, Guangzhou, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/6(2011-06-01), 559-566 1058-0468 28:6<559 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer