caa a22 4500 445816740 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9606-9 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9606-9 Intraindividual variability of homocysteine and related thiols concentrations in follicular fluid [Elektronische Daten] [Michaela Kralikova, Jitka Melounova, Igor Crha, Milena Matejovicova, Jana Zakova, Jiri Jarkovsky, Jaroslava Tallova] Purpose: To determine intraindividual variability in concentrations of homocysteine and related thiols in follicular fluids of particular follicles after ovarian stimulation and assess the differences between follicles with/or without oocytes. Methods: HPLC-FD analysis of plasma and follicular fluid cysteine, homocysteine, cysteinylglycine and glutathione in women undergoing IVF. Results: In blood plasma, the homocysteine, cysteine, and cysteinylglycine concentrations decreased significantly during stimulation with rec FSH (p < 0.001). We found significant differences in concentrations of cysteine and glutathione between follicles with or without retrieved oocytes. High intraindividual variability in concentrations of thiols was determined. Conclusions: The concentration variability of thiols between single follicles is very high and we recommend mean at least from 3 follicles with/or without oocytes for characterization of each woman. It is the best to examine individual follicles for further research and analysis of fertility outcomes. Springer Science+Business Media, LLC, 2011 Homocysteine nationallicence Thiols nationallicence Follicular fluid nationallicence Oocytes nationallicence Kralikova Michaela Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut Melounova Jitka Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut Crha Igor Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut Matejovicova Milena Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut Zakova Jana Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut Jarkovsky Jiri Institute of Biostatistics and Analyses, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut Tallova Jaroslava Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 863-868 1058-0468 28:9<863 2011 28 10815 https://doi.org/10.1007/s10815-011-9606-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9606-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kralikova Michaela Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Melounova Jitka Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Crha Igor Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Matejovicova Milena Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Zakova Jana Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Jarkovsky Jiri Institute of Biostatistics and Analyses, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Tallova Jaroslava Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, 625 00, Brno, Czech Republic aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 863-868 1058-0468 28:9<863 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer