caa a22 4500 445816759 CHVBK 20180317145230.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9602-0 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9602-0 Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker [Elektronische Daten] [Hsin-Chih Chao, Chia-Ling Chung, Hsien-An Pan, Pao-Chi Liao, Pao-Lin Kuo, Chao-Chin Hsu] Purpose: To understand the molecular basis of sperm-motility and to identify related novel motility biomarkers. Methods: Two-dimensional electrophoresis (2DE) followed by Reverse-phase-nano-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-nano-HPLC-ESI-MS/MS) were applied to establish the human sperm proteome. Then the sperm proteome of moderate-motile human sperm fraction and that of good-motile human sperm fraction from pooled spermatozoa of forty normozoospermic donors (Group 1 subjects) were compared to identify the dysregulated proteins. Among these down-regulated proteins, Protein tyrosine phosphatase non-receptor type 14 (PTPN14) was chosen to reconfirm by Western blotting and semi-quantitative reverse transcription polymerase chain reaction. For clinical application, Western blotting and real-time reverse transcription polymerase chain reaction was performed to compare the expression level of PTPN14 in (Group 2 subjects) nine normozoospermic controls and thirty-three asthenozoospermic patients (including 21 mild asthenozoospermic cases and 12 severe cases). Finally, bioinformatic tools prediction and immunofluorescence assay were performed to elucidate the potential localization of PTPN14. Results: The expression levels of three proteins were observed to be lower in the moderate-motile sperm fraction than in good-motile sperm of group 1 subjects. Among three proteins with persistent down-regulation in the moderate-motile sperm, we reconfirmed that the expression level of PTPN14 was significantly lower in both mRNA and protein levels from the moderate-motile sperm fraction. Further, down-regulation of PTPN14 was found at the translational and transcriptional level in the asthenozoospermic men. Finally, Bioinformatic tools prediction and immunofluorescence assay showed that PTPN14 maybe predominantly localized at the mitochondria in the midpiece of human ejaculated sperm. Conclusions: Proteomics tools were applied to identify three possible sperm motility-related proteins. Among these proteins, PTPN14 was highly likely a novel sperm-motility biomarker and a potential mitochondrial protein. Springer Science+Business Media, LLC, 2011 Protein tyrosine phosphatase nonreceptor 14 (PTPN14) nationallicence Proteomics nationallicence Sperm motility nationallicence Biomarker nationallicence Mitochondrial protein nationallicence Chao Hsin-Chih Division of Obstetrics and Gynecology, National Cheng Kung University College of Medicine and Hospital, Dou-Liou Branch, Yunlin, Taiwan aut Chung Chia-Ling Department of Obstetrics & Gynecology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan aut Pan Hsien-An Department of Obstetrics & Gynecology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan aut Liao Pao-Chi Department of Environmental and Occupational Medicine, National Cheng Kung University College of Medicine, Tainan, Taiwan aut Kuo Pao-Lin Department of Obstetrics & Gynecology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan aut Hsu Chao-Chin Department of Obstetrics & Gynecology, China Medical University, Taichung, Taiwan aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 851-861 1058-0468 28:9<851 2011 28 10815 https://doi.org/10.1007/s10815-011-9602-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9602-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chao Hsin-Chih Division of Obstetrics and Gynecology, National Cheng Kung University College of Medicine and Hospital, Dou-Liou Branch, Yunlin, Taiwan aut NATIONALLICENCE 700 1- Chung Chia-Ling Department of Obstetrics & Gynecology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan aut NATIONALLICENCE 700 1- Pan Hsien-An Department of Obstetrics & Gynecology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan aut NATIONALLICENCE 700 1- Liao Pao-Chi Department of Environmental and Occupational Medicine, National Cheng Kung University College of Medicine, Tainan, Taiwan aut NATIONALLICENCE 700 1- Kuo Pao-Lin Department of Obstetrics & Gynecology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan aut NATIONALLICENCE 700 1- Hsu Chao-Chin Department of Obstetrics & Gynecology, China Medical University, Taichung, Taiwan aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 851-861 1058-0468 28:9<851 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer