caa a22 4500 445816783 CHVBK 20180317145231.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9608-7 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9608-7 Preimplantation genetic screening: does it help or hinder IVF treatment and what is the role of the embryo? [Elektronische Daten] [Kim Ly, Ashok Agarwal, Zsolt Nagy] Despite an ongoing debate over its efficacy, preimplantation genetic screening (PGS) is increasingly being used to detect numerical chromosomal abnormalities in embryos to improve implantation rates after IVF. The main indications for the use of PGS in IVF treatments include advanced maternal age, repeated implantation failure, and recurrent pregnancy loss. The success of PGS is highly dependent on technical competence, embryo culture quality, and the presence of mosaicism in preimplantation embryos. Today, cleavage stage biopsy is the most commonly used method for screening preimplantation embryos for aneuploidy. However, blastocyst biopsy is rapidly becoming the more preferred method due to a decreased likelihood of mosaicism and an increase in the amount of DNA available for testing. Instead of using 9 to 12 chromosome FISH, a 24 chromosome detection by aCGH or SNP microarray will be used. Thus, it is advised that before attempting to perform PGS and expecting any benefit, extended embryo culture towards day 5/6 should be established and proven and the clinical staff should demonstrate competence with routine competency assessments. A properly designed randomized control trial is needed to test the potential benefits of these new developments. Springer Science+Business Media, LLC, 2011 Preimplantation genetic screening nationallicence Aneuploidy nationallicence In vitro fertilization nationallicence Embryo quality nationallicence Embryo biopsy nationallicence Ly Kim Center for Reproductive Medicine, Cleveland Clinic, Cleveland, USA aut Agarwal Ashok Center for Reproductive Medicine, Cleveland Clinic, Cleveland, USA aut Nagy Zsolt Reproductive Biology Associates, Atlanta, GA, USA aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 833-849 1058-0468 28:9<833 2011 28 10815 https://doi.org/10.1007/s10815-011-9608-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9608-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ly Kim Center for Reproductive Medicine, Cleveland Clinic, Cleveland, USA aut NATIONALLICENCE 700 1- Agarwal Ashok Center for Reproductive Medicine, Cleveland Clinic, Cleveland, USA aut NATIONALLICENCE 700 1- Nagy Zsolt Reproductive Biology Associates, Atlanta, GA, USA aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 833-849 1058-0468 28:9<833 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer