caa a22 4500 445816848 CHVBK 20180317145231.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9600-2 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9600-2 Women with high telomerase activity in luteinised granulosa cells have a higher pregnancy rate during in vitro fertilisation treatment [Elektronische Daten] [Hong Chen, Wenjun Wang, Yaqin Mo, Yun Ma, Nengyong Ouyang, Ruiqi Li, Meiqi Mai, Yingming He, M. Bodombossou-Djobo, Dongzi Yang] Objective: To study the effect of telomerase activity (TA) in human luteinised granulosa cells (GCs) on the outcome of in vitro fertilisation treatment. Methods: Fifty-six women, aged 23 to 39years, were enrolled and divided into four groups according to their levels of TA. Results: Seventeen cases in group A exhibited nondetectable TA, 16 cases in group B expressed low levels of TA (between 0.1 and 0.65 OD × mm), 14 cases in group C expressed moderate TA levels (between 0.66 and 1.00 OD × mm) and 9 cases in group D expressed high levels of TA (more than 1.00 OD × mm). The level of total serum testosterone (T) was significantly higher in groups C and D than in group A (1.43 ± 0.10 vs. 1.08 ± 0.11nmol/L, P < 0.030 and 1.56 ± 0.08 vs. 1.08 ± 0.11nmol/L, P < 0.005, respectively). The TA level was positively correlated with T (r = 0.291, P < 0.011). No obvious differences were observed in rates of fertilisation, cleavage, mature oocyte formation or good-quality embryos among the groups. The patients in group D exhibited the highest rates of embryo implantation and clinical pregnancy (with rates of 52.63% and 77.78%, respectively, compared with 18.92% and 29.41% in group A, 25.71% and 37.50% in group B and 48% and 50% in group C, with P < 0.018 and P = 0.112, respectively). The patients in group D also had a greater likelihood of becoming pregnant than those in group A (OR: 9.703, P < 0.023), group B (OR: 14.765, P < 0.009) or group C (OR: 5.560, P = 0.103). Conclusions: Luteinised GCs have a certain potential for proliferation and TA of luteinised GCs may predict the clinical outcomes of IVF treatment. Some unknown regulatory mechanisms between TA and T should be studied in further trials. Springer Science+Business Media, LLC, 2011 Telomerase activity nationallicence Ovarian granulosa cells nationallicence Controlled ovarian stimulation nationallicence In vitro fertilisation nationallicence Embryos transferred nationallicence Chen Hong Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Wang Wenjun Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Mo Yaqin Endocrinology laboratory of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Ma Yun Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Ouyang Nengyong Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Li Ruiqi Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Mai Meiqi Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut He Yingming Endocrinology laboratory of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Bodombossou-Djobo M. Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Yang Dongzi Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 797-807 1058-0468 28:9<797 2011 28 10815 https://doi.org/10.1007/s10815-011-9600-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9600-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Hong Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Wenjun Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Mo Yaqin Endocrinology laboratory of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ma Yun Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ouyang Nengyong Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Ruiqi Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Mai Meiqi Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- He Yingming Endocrinology laboratory of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Bodombossou-Djobo M. Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Dongzi Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Memorial Hospital of SUN YAT-SEN University, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/9(2011-09-01), 797-807 1058-0468 28:9<797 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer