caa a22 4500 445816899 CHVBK 20180317145231.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10815-011-9563-3 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9563-3 Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification [Elektronische Daten] [Jie Yan, Joao Suzuki, Xiao-Min Yu, Jie Qiao, Frederick Kan, Ri-Cheng Chian] Purpose: To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming. Methods: Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8-10days, 90-92days and 180-182days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared. Result(s): The oocyte cryo-survival rate declined following cryo-storage duration for 180-182days (90.4 ± 7.9%) compared to that of the other two groups (97.4 ± 3.0% and 98.0 ± 3.3%). The fertilization rate in the group of 180-182days (66.6 ± 22.0%) was also significantly reduced (P < 0.05) compared with the groups of 8-10days (92.2 ± 10.8%) and 90-92days (94.7 ± 9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P < 0.05) following long cryo-storage duration (72.1 ± 8.2%, 25.2 ± 3.8% and 5.5 ± 13.6%, respectively). Conclusion(s): The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen. Springer Science+Business Media, LLC, 2011 Oocyte nationallicence Cryo-storage nationallicence Vitrification nationallicence Liquid nitrogen nationallicence Cryo-survival nationallicence Yan Jie Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China aut Suzuki Joao Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Yu Xiao-Min Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Qiao Jie Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China aut Kan Frederick Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, Canada aut Chian Ri-Cheng Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/7(2011-07-01), 643-649 1058-0468 28:7<643 2011 28 10815 https://doi.org/10.1007/s10815-011-9563-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9563-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Jie Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China aut NATIONALLICENCE 700 1- Suzuki Joao Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Yu Xiao-Min Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Qiao Jie Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China aut NATIONALLICENCE 700 1- Kan Frederick Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, Canada aut NATIONALLICENCE 700 1- Chian Ri-Cheng Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/7(2011-07-01), 643-649 1058-0468 28:7<643 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer