caa a22 4500 445816902 CHVBK 20180317145231.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10815-011-9556-2 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9556-2 Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy [Elektronische Daten] [Li Zhang, Ahmet Yilmaz, Ri-Cheng Chian, Weon-Young Son, Xiao Zhang, Dewen Kong, Michael Dahan, Hannanel Holzer, Seang Tan, Asangla Ao] Purpose: To evaluate preimplantation genetic diagnosis (PGD) efficiency in thawed human embryos vitrified without biopsy. Methods: In this retrospective clinical study, 21 PGD cycles were carried out using fresh and vitrified-thawed embryos collected from 21 patients. Results: One hundred and ninety-nine embryos from patients with aneuploidy, single gene defects, or chromosomal translocations were vitrified at the cleavage stages; 93.5% of the embryos survived the vitrification procedure. Conclusive FISH results were obtained in 98.4% of the fresh and 99% of the frozen-thawed embryos screened for aneuploidy or chromosomal translocations. Conclusive PCR test results were obtained in 100% of the fresh and 93.9% of the frozen-thawed embryos screened for single gene defects. The overall clinical pregnancy rate per cycle was 57.1%. To date, 13 healthy children have been born. Conclusion: Reliable genetic diagnosis can be performed in thawed human embryos vitrified without biopsy. However, further research is required to support this conclusion. Springer Science+Business Media, LLC, 2011 Human nationallicence In vitro fertilization (IVF) nationallicence Preimplantation genetic diagnosis (PGD) nationallicence Vitrification nationallicence Zhang Li MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Yilmaz Ahmet MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Chian Ri-Cheng MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Son Weon-Young MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Zhang Xiao MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Kong Dewen MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Dahan Michael MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Holzer Hannanel MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Tan Seang MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Ao Asangla MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/7(2011-07-01), 597-602 1058-0468 28:7<597 2011 28 10815 https://doi.org/10.1007/s10815-011-9556-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9556-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Li MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Yilmaz Ahmet MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Chian Ri-Cheng MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Son Weon-Young MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Zhang Xiao MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Kong Dewen MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Dahan Michael MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Holzer Hannanel MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Tan Seang MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Ao Asangla MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/7(2011-07-01), 597-602 1058-0468 28:7<597 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer