caa a22 4500 445817003 CHVBK 20180317145231.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10815-011-9562-4 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9562-4 Fully-mature antral mouse oocytes are transcriptionally silent but their heterochromatin maintains a transcriptional permissive histone acetylation profile [Elektronische Daten] [Maurizio Zuccotti, Michele Bellone, Frank Longo, Carlo Redi, Silvia Garagna] Purpose: The final stages of antral mouse oocytes maturation are characterised by the transition from transcriptionally active NSN to inactive SN oocytes. Here, we studied the profile of histone acetylation changes occurring during the NSN-to-SN transition. Methods: During the NSN-to-SN transition, oocytes were classified based on their chromatin organisation and the immunocytochemical profile of histones H2B, H3 and H4 acetylation was analysed. Results: We described four patterns of immunostaining common to the three acetylated histones, corresponding to stages of progressive localisation: From a diffused distribution in NSN oocytes to the association with constitutive heterochromatin and then to the nucleolar surface region in SN oocytes. Conclusions: The maintenance of a favourable transcriptional epigenetic context, in the heterochromatin of transcriptionally silent SN oocytes, might be related to the early stages of development when transcripts from these heterochromatic regions are functional to preimplantation progression. Springer Science+Business Media, LLC, 2011 Mouse nationallicence Antral oocytes nationallicence Chromatin organisation nationallicence Histone acetylation nationallicence Zuccotti Maurizio Sezione di Istologia ed Embriologia, Dipartimento di Medicina Sperimentale, Universita' degli Studi di Parma, Parma, Italy aut Bellone Michele Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita' degli Studi di Pavia, Pavia, Italy aut Longo Frank Department of Anatomy, University of Iowa, Iowa, IA, USA aut Redi Carlo Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita' degli Studi di Pavia, Pavia, Italy aut Garagna Silvia Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita' degli Studi di Pavia, Pavia, Italy aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1193-1196 1058-0468 28:12<1193 2011 28 10815 https://doi.org/10.1007/s10815-011-9562-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9562-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zuccotti Maurizio Sezione di Istologia ed Embriologia, Dipartimento di Medicina Sperimentale, Universita' degli Studi di Parma, Parma, Italy aut NATIONALLICENCE 700 1- Bellone Michele Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita' degli Studi di Pavia, Pavia, Italy aut NATIONALLICENCE 700 1- Longo Frank Department of Anatomy, University of Iowa, Iowa, IA, USA aut NATIONALLICENCE 700 1- Redi Carlo Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita' degli Studi di Pavia, Pavia, Italy aut NATIONALLICENCE 700 1- Garagna Silvia Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita' degli Studi di Pavia, Pavia, Italy aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1193-1196 1058-0468 28:12<1193 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer