caa a22 4500 44581702X CHVBK 20180317145231.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10815-011-9650-5 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9650-5 Early cumulus cell removal could reduce the available embryo rate in human IVF [Elektronische Daten] [Duo Wei, Cuilian Zhang, Baoli Yin, Peng Wang, Juanke Xie, Xiaobing Song, Qi Liu, Lin Hu, Yixuan Zhang, Haoying Hao] Purpose: This study was designed to compare cumulus cell removal at different times and to evaluate their effects on embryo developmental potential and the outcomes of in vitro fertilization (IVF). Methods: We retrieved 606 IVF cycles with standard long down regulation protocol from January 2010 to December 2010. These cycles were divided into two groups: group A, 364 cycles with high risk of fertilization failure, whose cumulus cells were removed 4h after short gamete coincubation; group B, 242 cycles as control, whose cumulus cells removal were performed 17 to 18h after insemination. The epidemiological, clinical, laboratory factors and the outcomes of these cycles were analyzed. Results: The available embryo rate of group A was significantly lower (P = 0.002). There were no significant differences in other laboratory parameters and outcomes between the two groups (P > 0.05). Conclusions: For patients with high risk of fertilization failure, there could be a risk of compromising the rate of available embryos, if fertilization is judged by the presence of 2PB by cumulus cell removal only 4h post-insemination. Therefore, this strategy is not recommended to all IVF cycles and future studies are needed to confirm its reliability. Springer Science+Business Media, LLC, 2011 Short gamete coincubation nationallicence Fertilization nationallicence Cumulus cell nationallicence Available embryo rate nationallicence In vitro fertilization nationallicence Wei Duo The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Zhang Cuilian The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Yin Baoli The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Wang Peng National Research Center for Assisted Reproductive Technology and Reproductive Genetics of China; The Key laboratory for reproductive Endocrinology of Ministry of Education; Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 250021, Shandong, China aut Xie Juanke The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Song Xiaobing The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Liu Qi The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Hu Lin The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Zhang Yixuan The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Hao Haoying The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1213-1216 1058-0468 28:12<1213 2011 28 10815 https://doi.org/10.1007/s10815-011-9650-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9650-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Duo The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Zhang Cuilian The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Yin Baoli The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Wang Peng National Research Center for Assisted Reproductive Technology and Reproductive Genetics of China; The Key laboratory for reproductive Endocrinology of Ministry of Education; Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 250021, Shandong, China aut NATIONALLICENCE 700 1- Xie Juanke The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Song Xiaobing The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Liu Qi The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Hu Lin The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Zhang Yixuan The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Hao Haoying The Reproductive Medical Center of Henan Provincial People's Hospital, NO.7 Weiwu Road, 450003, Zhengzhou, Henan, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1213-1216 1058-0468 28:12<1213 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer