caa a22 4500 445817038 CHVBK 20180317145231.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10815-011-9630-9 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9630-9 Effects of cumulus cells removal after 6 h co-incubation of gametes on the outcomes of human IVF [Elektronische Daten] [Shun Xiong, Wei Han, Jun Liu, Xiao Zhang, Wei Liu, Hong Liu, Guo Huang] Purpose: To investigate the effects of cumulus cells removal after 6h co-incubation of gametes on the fertilization, polyspermy, multinucleation and clinical pregnancy rates in human IVF. Methods: A total of 1,200 IVF-ET cycles undergoing 6h co-incubation of gametes in 2009 were included in this study. Inclusion criteria were: female age <38years, first IVF treatment, with bi-ovary and normal ovarian response, e.g., 4 ~ 20 oocytes could be obtained. A 6h period of co-incubation was applied in all IVF cycles. According to the history of infertility, cumulus cells were mechanically removed either 6h post-insemination or 20h post-insemination. For couples with primary infertility, or unexplained infertility, or mild oligospermia or asthenospermia, the cumulus cells were removed at 6h of insemination for the polar body observation (6h group, n = 565). Of these, 80 cycles received early rescue ICSI due to fertilization failure or low fertilization rate at 6h of insemination. For couples with secondary infertility and normal semen analysis, the cumulus cells were removed at 20h of insemination as routine (20h group, n = 635). Of these, three cycles received late rescue ICSI due to fertilization failure at 20h of insemination. Normal fertilization, polyspermy (≥3PN), multinucleation and clinical pregnancy rates were compared between the two groups (rescue ICSI cycles were not included in the comparison in both groups). Results: Significant difference (P < 0.05) was observed between the groups regarding polyspermy rates (7.48% in 6h group and 9.22% in 20h group). No difference was observed between the groups regarding normal fertilization rates (2PN rate) (64.89% in 6h group and 65.74% in 20h group). No difference was observed between the groups regarding multinucleation and clinical pregnancy rates (11.01% and 65.15% in 6h group, 10.75% and 66.93% in 20h group, respectively). The clinical pregnancy rate was 51.43% in cycles receiving early rescue ICSI, while no clinical pregnancy was obtained in cycles receiving late rescue ICSI. Conclusion: The present results indicate that cumulus cells removal at 6h of insemination is a relatively safe operation, which yielded comparable normal fertilization rate, multinucleation and clinical pregnancy rates compared with 20h group. This protocol may be beneficial for early obsevation of fertilization failure and make early rescue ICSI possible. Springer Science+Business Media, LLC, 2011 In-vitro fertilization nationallicence Short co-incubation nationallicence Cumulus cells removal nationallicence Polyspermy nationallicence Clinical pregnancy nationallicence Rescue ICSI nationallicence Xiong Shun Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut Han Wei Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut Liu Jun Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut Zhang Xiao Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut Liu Wei Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut Liu Hong Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut Huang Guo Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1205-1211 1058-0468 28:12<1205 2011 28 10815 https://doi.org/10.1007/s10815-011-9630-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9630-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiong Shun Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut NATIONALLICENCE 700 1- Han Wei Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut NATIONALLICENCE 700 1- Liu Jun Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut NATIONALLICENCE 700 1- Zhang Xiao Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut NATIONALLICENCE 700 1- Liu Wei Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut NATIONALLICENCE 700 1- Liu Hong Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut NATIONALLICENCE 700 1- Huang Guo Chong Qing Reproductive and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, 64 Jing Tang ST, 400013, Chongqing, Yu Zhong District, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1205-1211 1058-0468 28:12<1205 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer