caa a22 4500 445817062 CHVBK 20180317145231.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10815-011-9649-y doi (NATIONALLICENCE)springer-10.1007/s10815-011-9649-y Viability assessment of fresh and frozen/thawed isolated human follicles: reliability of two methods (Trypan blue and Calcein AM/ethidium homodimer-1) [Elektronische Daten] [Sandra Sanfilippo, Michel Canis, Lemlih Ouchchane, Revaz Botchorishvili, Christine Artonne, Laurent Janny, Florence Brugnon] Purpose: To assess the reliability of trypan blue (TB) and calcein AM/ethidium homodimer-1 (CaAM/EthD-1) staining to evaluate the viability of fresh and thawed human ovarian follicles. Methods: Isolated follicles from fresh and thawed cortex were stained using TB versus CaAM/EthD-1 methods (n = 10 patients). Measurements were performed by two independent observers. The reliability was evaluated by intraclass correlation coefficient (ICC) and the differences between paired measurements were tested by the Wilcoxon test. Results: Inter-observer reliability was excellent for each method. Nevertheless, it was even better with the TB method (ICC = 0.83) compared with CaAM/EthD-1 (ICC = 0.75). Moreover, the ICCs for viability measurements using the two methods were good for each observer (observer 1: ICC = 0.49; observer 2: ICC = 0.40). Conclusion: Compared with CaAM/EthD-1, TB appears to be more reliable as a staining method for follicle viability evaluation. TB staining is a quick and useful method, complementary to histological analysis for quality control in ovarian tissue cryopreservation. Springer Science+Business Media, LLC, 2011 Human ovarian tissue nationallicence Fresh/thawed isolated follicles nationallicence Viability staining nationallicence Reliability nationallicence Trypan blue nationallicence Calcein AM/ethidium homodimer-1 nationallicence Sanfilippo Sandra Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut Canis Michel Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut Ouchchane Lemlih Laboratoire de biostatistiques, télématique et traitement d'image, Université Clermont 1, UFR Médecine, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut Botchorishvili Revaz Centre International de Chirurgie Endoscopique (CICE), 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut Artonne Christine Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut Janny Laurent Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut Brugnon Florence Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1151-1156 1058-0468 28:12<1151 2011 28 10815 https://doi.org/10.1007/s10815-011-9649-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9649-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sanfilippo Sandra Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut NATIONALLICENCE 700 1- Canis Michel Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut NATIONALLICENCE 700 1- Ouchchane Lemlih Laboratoire de biostatistiques, télématique et traitement d'image, Université Clermont 1, UFR Médecine, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut NATIONALLICENCE 700 1- Botchorishvili Revaz Centre International de Chirurgie Endoscopique (CICE), 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut NATIONALLICENCE 700 1- Artonne Christine Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut NATIONALLICENCE 700 1- Janny Laurent Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut NATIONALLICENCE 700 1- Brugnon Florence Laboratoire de biologie du développement et de la reproduction, Université Clermont 1, UFR Médecine, E.A. 975, 28 Place Henri Dunant, 63001, Clermont-Ferrand, Cedex 1, France aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1151-1156 1058-0468 28:12<1151 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer