caa a22 4500 445817097 CHVBK 20180317145232.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10815-011-9672-z doi (NATIONALLICENCE)springer-10.1007/s10815-011-9672-z Impact of freezing and thawing of human ovarian tissue on follicular growth after long-term xenotransplantation [Elektronische Daten] [Christiani Amorim, Anu David, Marie-Madeleine Dolmans, Alessandra Camboni, Jacques Donnez, Anne Van Langendonckt] Purpose: To assess follicular growth after xenografting in order to understand how freezing and/or grafting may affect follicular development. Methods: Human ovarian biopsies were used for fresh and frozen-thawed xenografting to SCID mice. After xenotransplantation, follicular morphology and proportion, oocyte and follicle diameter, and quantitative and qualitative parameters of antral follicles were analyzed. Results: The proportion of growing follicles was significantly higher in grafted than non-grafted ovarian tissue. Follicular growth to the antral stage was observed and there was no significant difference in oocyte or follicle diameter in fresh or frozen-thawed grafts. Although no significant difference was observed in antral area or zona pellucida thickness, the theca layer in antral follicles from frozen-thawed grafted tissue was found to be significantly thinner than in fresh grafts. Conclusion: Antral follicles obtained after grafting of frozen-thawed human ovarian tissue showed a thinner theca cell layer compared to those from fresh grafts, which could affect follicular development and function. Further studies are nevertheless warranted to confirm the identity of theca cells and assess if they retain the ability to respond to luteinizing hormone and produce androgens. Springer Science+Business Media, LLC, 2011 Ovarian tissue nationallicence Follicles nationallicence Xenotransplantation nationallicence Cryopreservation nationallicence Theca cell layer nationallicence Amorim Christiani Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut David Anu Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut Dolmans Marie-Madeleine Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut Camboni Alessandra Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut Donnez Jacques Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut Van Langendonckt Anne Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1157-1165 1058-0468 28:12<1157 2011 28 10815 https://doi.org/10.1007/s10815-011-9672-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9672-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amorim Christiani Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut NATIONALLICENCE 700 1- David Anu Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Dolmans Marie-Madeleine Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Camboni Alessandra Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Donnez Jacques Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut NATIONALLICENCE 700 1- Van Langendonckt Anne Department of Gynecology, Université Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, 1200, Brussels, Belgium aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/12(2011-12-01), 1157-1165 1058-0468 28:12<1157 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer