caa a22 4500 445817194 CHVBK 20180317145232.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10815-010-9503-7 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9503-7 There is no complete linkage between the polymorphisms N680S and T307A of the follicular stimulating hormone receptor gene in fertile women [Elektronische Daten] [Gustavo Rodini, Vanessa Genro, Ursula Matte, Fernanda Pereira, João Bilibio, Camila Greggianin, Carlos Souza, João Cunha-Filho] Purpose: We conducted a cross-sectional study to evaluate the linkage of FSHR T307A and N680S in a group of fertile women. Methods: Peripheral blood was obtained from 51 fertile women. DNA extraction and isolation were performed. For the detection of the T307A polymorphism a set of primers (5_-TCTGAGCTTCATCCAATTTGCA-3_and 5_-GGGAAAGAGGGCA GCTGCAA-3) was used and then the product was further amplified by a second PCR-RFLP using another set of primers (5_-CAAATCTATTTTAAGGCAAGAAGTTGATTATATGCCTCAG-3_and 5_-GTAGATTCCAATGCAGA GATCA-3). For the N680S polymorphism the primers (5_-TTTGTGGTCATCTGTGGCTGC-3_ and 5_-CAAAGGCAAGGACTGAATT ATC ATT-3_) were used. Statistical analysis for the association between the polymorphisms was performed by the Spearman test. Results: We calculated the association between the homozygosis at codon 307 and at codon 680 both for T/T-S/S and A/A-N/N. A significant association between the genotypic results at codon 680 with those at codon 307 was found (r = 0.6363, P = 0.001). However, a complete linkage between these two polymorphisms was rejected as there were 12 patients with discordant results from the expected A-N/T-S at codons 307 and 680, respectively. Conclusion: The current data demonstrated an association but failed to demonstrate a complete linkage between these two polymorphisms. Springer Science+Business Media, LLC, 2010 FSH polymorphisms nationallicence Fertile women nationallicence Linkage nationallicence FSH receptor gene nationallicence Rodini Gustavo Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Genro Vanessa Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Matte Ursula Terapia Genica Research Unit, Research Center of Hospital de Clinicas de Porto Alegre, Porto Alegre, Brazil aut Pereira Fernanda Terapia Genica Research Unit, Research Center of Hospital de Clinicas de Porto Alegre, Porto Alegre, Brazil aut Bilibio João Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Greggianin Camila Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Souza Carlos Department of Obstetrics and Gynecology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Cunha-Filho João Department of Obstetrics and Gynecology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/3(2011-03-01), 221-224 1058-0468 28:3<221 2011 28 10815 https://doi.org/10.1007/s10815-010-9503-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9503-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodini Gustavo Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Genro Vanessa Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Matte Ursula Terapia Genica Research Unit, Research Center of Hospital de Clinicas de Porto Alegre, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Pereira Fernanda Terapia Genica Research Unit, Research Center of Hospital de Clinicas de Porto Alegre, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Bilibio João Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Greggianin Camila Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Souza Carlos Department of Obstetrics and Gynecology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Cunha-Filho João Department of Obstetrics and Gynecology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/3(2011-03-01), 221-224 1058-0468 28:3<221 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer