caa a22 4500 445817216 CHVBK 20180317145232.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10815-010-9505-5 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9505-5 Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa [Elektronische Daten] [Martin Wilding, Gianfranco Coppola, Loredana di Matteo, Antonio Palagiano, Enrico Fusco, Brian Dale] Purpose: We used computer assisted sperm selection (MSOME) during cycles of intracytoplasmic sperm injection to test whether this technique improves results over traditional ICSI protocols. We also used the TUNEL assay to test whether MSOME could deselect physiologically abnormal spermatozoa. Methods: Individual spermatozoa were examined with MSOME. Normal and abnormal spermatozoa were tested for the level of DNA fragmentation using TUNEL assay. In a prospective, randomized trial, patients were selected for standard ICSI, or IMSI techniques. We tested the two groups for biological and clinical parameters. Results: 64.8% of spermatozoa, otherwise selectable for ICSI, were characterized by abnormalities after computer-assisted sperm analysis. These sperm were also characterized by an increase in the level of DNA fragmentation. We noted an increase in embryo quality, pregnancy and implantation rates after computerized sperm selection during ICSI procedures. Conclusions: Computerised selection of spermatozoa during ICSI procedures deselects physiological abnormal spermatozoa and improves clinical results. Springer Science+Business Media, LLC, 2010 MSOME nationallicence IMSI nationallicence TUNEL assay nationallicence ICSI nationallicence Human embryo nationallicence Wilding Martin Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut Coppola Gianfranco Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut di Matteo Loredana Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut Palagiano Antonio Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut Fusco Enrico Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut Dale Brian Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/3(2011-03-01), 253-262 1058-0468 28:3<253 2011 28 10815 https://doi.org/10.1007/s10815-010-9505-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9505-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wilding Martin Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut NATIONALLICENCE 700 1- Coppola Gianfranco Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut NATIONALLICENCE 700 1- di Matteo Loredana Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut NATIONALLICENCE 700 1- Palagiano Antonio Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut NATIONALLICENCE 700 1- Fusco Enrico Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut NATIONALLICENCE 700 1- Dale Brian Centro Fecondazione Assistita, Clinica Villa del Sole, Via Manzoni 15, 80122, Napoli, Italy aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/3(2011-03-01), 253-262 1058-0468 28:3<253 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer