caa a22 4500 445817321 CHVBK 20180317145232.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9609-6 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9609-6 Relative expression of genes encoding SMAD signal transduction factors in human granulosa cells is correlated with oocyte quality [Elektronische Daten] [Fang-Ting Kuo, Kenneth Fan, Gayane Ambartsumyan, Priya Menon, Aline Ketefian, Ikuko Bentsi-Barnes, Margareta Pisarska] Purpose: To determine the expression of SMAD transcripts in human granulosa cells. Methods: Luteinized mural granulosa cells were harvested from forty women undergoing oocyte retrieval, and RNAs were isolated. SMAD expression levels were determined by polymerase chain reaction (PCR) and quantitative real-time PCR (q-RTPCR). Results: SMAD1-7 and 9 are expressed in human granulosa cells, with SMAD2, 3 and 4 showing the highest expression levels. Peak estradiol (E2) levels correlated with the number of oocytes retrieved during IVF. Oocyte number showed no correlation with SMAD expression levels or ratios. Fertilization rates also did not correlate with the expression levels of individual SMADs, but did correlate with higher SMAD4:SMAD3 ratios (p = 0.0062) and trended with SMAD4:SMAD2 (p = 0.0698). Conclusions: SMAD transcripts are differently expressed in human granulosa cells, where they may mediate TGF-beta superfamily signaling during folliculogenesis and ovulation. Further, the relative expression ratios of SMAD2, 3 and 4 may differentially affect fertilization rate. Springer Science+Business Media, LLC, 2011 SMAD nationallicence Signal transduction nationallicence TGF-beta nationallicence Ovary nationallicence Granulosa cell nationallicence Human gene expression nationallicence Kuo Fang-Ting Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut Fan Kenneth Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut Ambartsumyan Gayane Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut Menon Priya David Geffen School of Medicine, UCLA, 405 Hilgard Avenue, Los Angeles, CA, USA aut Ketefian Aline Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut Bentsi-Barnes Ikuko Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut Pisarska Margareta Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 931-938 1058-0468 28:10<931 2011 28 10815 https://doi.org/10.1007/s10815-011-9609-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9609-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuo Fang-Ting Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Fan Kenneth Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Ambartsumyan Gayane Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Menon Priya David Geffen School of Medicine, UCLA, 405 Hilgard Avenue, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Ketefian Aline Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Bentsi-Barnes Ikuko Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Pisarska Margareta Center for Fertility and Reproductive Medicine, Division of REI, Department of Ob/Gyn, Cedars-Sinai Medical Center, 8635 West Third Street, Suite 160 W, 90048, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 931-938 1058-0468 28:10<931 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer