caa a22 4500 44581733X CHVBK 20180317145232.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9620-y doi (NATIONALLICENCE)springer-10.1007/s10815-011-9620-y Altitude can alter the mtDNA copy number and nDNA integrity in sperm [Elektronische Daten] [Yongjun Luo, Weigong Liao, Yu Chen, Jianhua Cui, Fuyu Liu, Chunhua Jiang, Wenxiang Gao, Yuqi Gao] Objective: Mitochondria are factories for energy production and genetic alterations in mtDNA will directly impact OXPHOS function. The copy number of mtDNA (i.e., the number of mtDNA per spermatozoon) is one of the major mitochondrial genetic features. Besides mtDNA copy number, the change of either mtDNA or nDNA integrity is another important factor causing asthenospermia, or poor sperm motility in infertile men. In this study, we investigated the mtDNA copy number and the integrities of mtDNA and nDNA respectively in semen samples from different donors at 5,300m altitudes. Methods: Total DNA was extracted from semen samples from donors in two different altitudes. Quantitative PCR was performed to evaluate the mtDNA copy number. PCR amplification was used to examine the integrity of sperm mtDNA. Flow cytometry was carried out to investigate sperm nDNA integrity. All data were analyzed to show the statistical significance. Results: Sperm mtDNA copy number for those living at high altitude (5,300m) for one month was significantly higher (P < 0.05) than for those at the lower altitude (1,400m) or in donors who had been living at the 5,300m altitude for 1year. In addition, sperm mtDNA copy numbers were remarkably decreased (P < 0.05) in those who had lived at the greater altitude for 1year compared to those who had lived there for one month. The ratio of nDNA integrity among the 10,000 sperms at high altitude for one month was significantly lower (P < 0.05) than that at the lower altitude (1,400m) or at 5,300m for 1year, and the ratio of nDNA integrity sperms at high altitude for 1year was increased, and higher than that for at the lower altitude (P < 0.05). Conclusion: High altitude can alter the mtDNA copy number and nDNA integrity in the sperms. Springer Science+Business Media, LLC, 2011 High altitude nationallicence Sperm nationallicence mtDNA nationallicence nDNA nationallicence Copy number nationallicence Integrity nationallicence Luo Yongjun Department of High Altitude Disease, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut Liao Weigong Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut Chen Yu Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut Cui Jianhua The 18th Hospital of the PLA, 844900, Yecheng, Xinjiang, China aut Liu Fuyu Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut Jiang Chunhua Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut Gao Wenxiang Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut Gao Yuqi Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 951-956 1058-0468 28:10<951 2011 28 10815 https://doi.org/10.1007/s10815-011-9620-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9620-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Yongjun Department of High Altitude Disease, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Liao Weigong Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Chen Yu Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Cui Jianhua The 18th Hospital of the PLA, 844900, Yecheng, Xinjiang, China aut NATIONALLICENCE 700 1- Liu Fuyu Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Jiang Chunhua Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Gao Wenxiang Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Gao Yuqi Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 951-956 1058-0468 28:10<951 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer