caa a22 4500 445817348 CHVBK 20180317145232.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9611-z doi (NATIONALLICENCE)springer-10.1007/s10815-011-9611-z Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization [Elektronische Daten] [Leandros Lazaros, Georgios Vartholomatos, Elissavet Hatzi, Apostolos Kaponis, Georgios Makrydimas, Sophia Kalantaridou, Nikolaos Sofikitis, Theodoros Stefos, Konstantinos Zikopoulos, Ioannis Georgiou] Purpose: Sperm flow cytometry (SFC) was used to evaluate the association of sperm chromatin condensation and ploidy with fertilization, embryo development, pregnancy and abortion rates following IVF. Methods: Conventional semen analysis was performed in one hundred fifty men, as well as SFC analysis, after acridine orange and propidium iodide staining, for the evaluation of sperm maturity and ploidy respectively. Conventional IVF was performed in all couples. Results: Couples with low percentages of mature spermatozoa presented with lower fertilization rates (p < 0.005), lower rates of grade A embryos (p < 0.003) and lower pregnancy rates (p < 0.006), compared to couples with high percentages of mature spermatozoa. Couples with low total aneuploidy rates presented with higher fertilization rates (p < 0.007), higher rates of grade A embryos (p < 0.004) and higher pregnancy rates (p < 0.003), compared to couples with high total aneuploidy rates. Conclusions: Sperm chromatin condensation and ploidy constitute critical parameters for the evaluation of semen samples before IVF and for the identification of cases in need of ICSI application. Springer Science+Business Media, LLC, 2011 Flow cytometry nationallicence IVF outcome nationallicence Pregnancy rate nationallicence Sperm chromatin condensation nationallicence Sperm ploidy status nationallicence Lazaros Leandros Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Vartholomatos Georgios Laboratory of Hematology - Unit of Molecular Biology, Ioannina University Hospital, Ioannina, Greece aut Hatzi Elissavet Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Kaponis Apostolos Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Makrydimas Georgios Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Kalantaridou Sophia Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Sofikitis Nikolaos Department of Urology, Medical School, University of Ioannina, Ioannina, Greece aut Stefos Theodoros Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Zikopoulos Konstantinos Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Georgiou Ioannis Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 885-891 1058-0468 28:10<885 2011 28 10815 https://doi.org/10.1007/s10815-011-9611-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9611-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lazaros Leandros Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 700 1- Vartholomatos Georgios Laboratory of Hematology - Unit of Molecular Biology, Ioannina University Hospital, Ioannina, Greece aut NATIONALLICENCE 700 1- Hatzi Elissavet Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 700 1- Kaponis Apostolos Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 700 1- Makrydimas Georgios Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 700 1- Kalantaridou Sophia Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 700 1- Sofikitis Nikolaos Department of Urology, Medical School, University of Ioannina, Ioannina, Greece aut NATIONALLICENCE 700 1- Stefos Theodoros Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 700 1- Zikopoulos Konstantinos Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 700 1- Georgiou Ioannis Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 885-891 1058-0468 28:10<885 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer