caa a22 4500 445817364 CHVBK 20180317145232.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10815-011-9621-x doi (NATIONALLICENCE)springer-10.1007/s10815-011-9621-x Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia [Elektronische Daten] [Soumaya Mougou-Zerelli, Sonia Brahem, Molka Kammoun, Mehdi Jerbi, Hatem Elghezal, Mounir Ajina, Ali Saad] Purpose: To evaluate the frequency of sperm nuclei disomy for chromosomes 8, X, and Y in patients with severe non-obstructive oligozoospermia and to assess possible correlations between sperm nuclei aneuploidy and semen parameters or a particular clinical phenotype. Materials and methods: The sperm aneuploidy rate for chromosomes X, Y, and 8 were assessed in 16 infertile men with severe non-obstructive oligozoospermia and 7 healthy men with normal semen parameters. The frequency of sperm aneuploidy was compared between several patients groups according to their clinical and biological factors. Results: The total rate of chromosomally abnormal spermatozoa was significantly higher in patients with severe oligozoospermia compared to control group (P < 0.001). A significant relationship was found between the age of patients, sperm concentration, and morphology and the mean rate of sex chromosomes disomy. In addition to the low sperm count (<5 × 106/ml), an elevated FSH level and an exposed to an elevated temperature are two major predictive factors leading to the production of higher numbers of chromosomally abnormal gametes. Conclusion: Patients with severe oligozoospermia, who are potential candidates for assisted reproduction technology, presented a high level of sex numerical chromosome abnormalities, and consequently are at high risk of chromosome abnormalities in their offspring. Springer Science+Business Media, LLC, 2011 Aneuploidy nationallicence FISH nationallicence Predictive factors nationallicence Severe oligozoospermia nationallicence Mougou-Zerelli Soumaya Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Brahem Sonia Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Kammoun Molka Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Jerbi Mehdi Medical Office of Gynaecology and Obstetrics, Avenue M. Karoui, 4000, Sousse, Tunisia aut Elghezal Hatem Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Ajina Mounir Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Saad Ali Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 971-977 1058-0468 28:10<971 2011 28 10815 https://doi.org/10.1007/s10815-011-9621-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9621-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mougou-Zerelli Soumaya Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Brahem Sonia Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Kammoun Molka Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Jerbi Mehdi Medical Office of Gynaecology and Obstetrics, Avenue M. Karoui, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Elghezal Hatem Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Ajina Mounir Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Saad Ali Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/10(2011-09-01), 971-977 1058-0468 28:10<971 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer