caa a22 4500 445817453 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10815-010-9518-0 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9518-0 A prospective randomized sibling-oocyte study of two media systems for culturing cleavage-stage embryos—impact on fertilization rate [Elektronische Daten] [Fredwell Hambiliki, Petra Sandell, Fatma Yaldir, Anneli Stavreus-Evers] Purpose: Although several media systems have been developed, data from prospective randomised clinical studies are still lacking. In the present study we compared the effects of 2 different media systems on embryo morphology and development at days 2/3 using sibling oocytes. Methods: In this prospective sibling-split trial, 1206 oocytes from 110 women were divided via alternate allocation to fertilization and culture in media system A (G-IVF TM v5 PLUS/ G-1TM v5 PLUS) or for fertilization and culture in media system B (Universal IVF medium/EmbryoAssist TM). Results: The use of media system A significantly increased the normal fertilization rate (73.5% versus 67.2%; p = 0.030) and embryo utilization rate (55.5% versus 42.9%; p = 0.001), whereas polyploidy and embryo quality were similar in the two groups. Conclusion: The different impacts on fertilization and early embryo development between the two commercially available and commonly used media systems show the importance of evaluation of the efficacy of existing sequential culture media and the need to further improve media for in vitro development of human embryos. Springer Science+Business Media, LLC, 2010 Sibling oocytes nationallicence Culture media nationallicence Fertilization rate nationallicence Hambiliki Fredwell Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynaecology, Karolinska Institutet, 141 86, Stockholm, Sweden aut Sandell Petra Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden aut Yaldir Fatma Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden aut Stavreus-Evers Anneli Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 335-341 1058-0468 28:4<335 2011 28 10815 https://doi.org/10.1007/s10815-010-9518-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9518-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hambiliki Fredwell Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynaecology, Karolinska Institutet, 141 86, Stockholm, Sweden aut NATIONALLICENCE 700 1- Sandell Petra Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Yaldir Fatma Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Stavreus-Evers Anneli Department of Women's and Children's Health, Uppsala University, 751 85, Uppsala, Sweden aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 335-341 1058-0468 28:4<335 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer